Methods for obtaining and analyzing images
First Claim
1. A method for assessing at least two adjacent sections A and B of a specimen, said method comprising the steps of:
- a) staining section A for one marker specific for one object type in the specimen, and staining section B for another marker specific for another object type in the specimen,b) obtaining an image of at least part of section A and an image of at least part of section B,c) carrying out an image registration process on the two images by establishing correspondence between features of the two related images obtaining a representation of a corresponding image pair,d) assessing marker events for at least two object types on said representation, ande) assessing the at least two adjacent sections A and B with respect to the two object types.
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Abstract
Disclosed is a method and a system for obtaining and analyzing image pairs, obtained as sections of specimen. The invention facilitates registration of two corresponding images, one from each section of the specimen. The invention includes performing a registration process of the two images thereby obtaining a mathematical transformation rule and afterwards using said transformation rule for each image field identified in one image allowing that the corresponding image field in the other image may be identified as well. After the corresponding image pairs have been obtained using the method of the present invention, the sections can be assessed, such as by identifying the counting events for at least one type of object on the image fields within at least one corresponding image pair, optionally using automatic means.
24 Citations
33 Claims
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1. A method for assessing at least two adjacent sections A and B of a specimen, said method comprising the steps of:
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a) staining section A for one marker specific for one object type in the specimen, and staining section B for another marker specific for another object type in the specimen, b) obtaining an image of at least part of section A and an image of at least part of section B, c) carrying out an image registration process on the two images by establishing correspondence between features of the two related images obtaining a representation of a corresponding image pair, d) assessing marker events for at least two object types on said representation, and e) assessing the at least two adjacent sections A and B with respect to the two object types. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33)
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Specification