Amperometric sensor and method for its manufacturing
First Claim
1. An in vivo amperometric biosensor for implantation into a human or animal to generate a signal corresponding to analyte concentration in a body fluid of the human or animal, the biosensor comprising:
- a support member;
working and counter electrodes arranged on the support member; and
a sensing layer with immobilized enzyme distributed therein, the sensing layer at least partially covering the working electrode;
wherein, the sensing layer has an effective diffusion coefficient such that, when the biosensor is implanted for use, diffusion of the analyte in the sensing layer is the rate-limiting step in generating the signal corresponding to analyte concentration.
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Abstract
An in vivo amperometric sensor is provided for measuring the concentration of an analyte in a body fluid. The sensor comprises a counter electrode and a working electrode, and the working electrode comprises a sensing layer which is generally water permeable and arranged on a support member adjacent to a contact pad. The sensing layer comprises an immobilized enzyme capable of acting catalytically in the presence of the analyte to cause an electrical signal. The sensing layer has an upper surface facing the body fluid and a lower surface facing away from the body fluid, and the immobilized enzyme is distributed within the sensing layer in such a way that the enzyme concentration in the middle between the upper and lower surfaces is at least as high as on the upper surface of the sensing layer.
20 Citations
9 Claims
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1. An in vivo amperometric biosensor for implantation into a human or animal to generate a signal corresponding to analyte concentration in a body fluid of the human or animal, the biosensor comprising:
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a support member; working and counter electrodes arranged on the support member; and a sensing layer with immobilized enzyme distributed therein, the sensing layer at least partially covering the working electrode; wherein, the sensing layer has an effective diffusion coefficient such that, when the biosensor is implanted for use, diffusion of the analyte in the sensing layer is the rate-limiting step in generating the signal corresponding to analyte concentration. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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Specification