Reagent holder, and kits containing same

• US 9,701,957 B2
• Filed: 07/14/2008
• Issued: 07/11/2017
• Est. Priority Date: 07/13/2007
• Status: Active Grant

• Current Assignee: HandyLab Incorporated (Becton, Dickinson & Co)
• Original Assignee: HandyLab Incorporated (Becton, Dickinson & Co)
• Inventors: Wilson, Kerry, Handique, Kalyan, Brahmasandra, Sundaresh N., Williams, Jeff
• Primary Examiner(s): Pregler, Sharon
• Application Number:
  US12/218,416
• Publication Number:
  US 20090129978A1
• Time in Patent Office: 3,284 Days
• Field of Search: 422 61, 422 63, 422 65, 422 99, 422100, 435975
• CPC Class Codes: C07C 233/36 having the carbon atom of the carboxamide group bound to a hydrogen atom or to a carbon atom of an acyclic saturated carbon skeleton
• CPC Class Codes: C07H 1/08 from natural products
• CPC Class Codes: C08G 83/003 Dendrimers
• CPC Class Codes: C12N 15/1013 by using magnetic beads
• CPC Class Codes: C12Q 1/6813 Hybridisation assays

Abstract

A holder for reagents, such as may be used for transporting the reagents and for carrying out processing operations on biological samples with the reagents. The holders typically hold reagents for amplifying polynucleotides extracted from the samples. The holder comprises a connecting member; a process tube affixed to the connecting member and having an aperture located in the connecting member; at least one socket, located in the connecting member, and configured to accept a pipette tip; two or more reagent tubes disposed on the underside of the connecting member, each having an inlet aperture located in the connecting member; and one or more receptacles, located in the connecting member and each being configured to receive a reagent tube. Also described are reagent tubes configured with stellated shaped patterns, on their bottom interior surfaces, configured to facilitate complete or near-complete withdrawal of fluid from the tube, via a pipette tip.

Claims

1. A unitized reagent holder and reagent system, comprising:

a strip, to which is attached;

a single process tube;

at least three reagent tubes;

a sample lysis reagent in a first said reagent tube;

a PCR reagent for a first analyte in a second said reagent tube;

one or more liquid reagents in a third said reagent tube; and

two or more sockets holding two or more pipette tips, wherein the ratio of sockets holding pipette tips to process tubes is at least two.

2. The unitized reagent holder of claim 1, further comprising a laminate that seals closed an inlet of each of the containers.

3. The unitized reagent holder of claim 1, wherein at least one of the containers is removable.

4. The unitized reagent holder of claim 1, further comprising a pipette tip sheath configured to surround one of the pipette tips.

5. The unitized reagent holder of claim 1, further comprising a waste tube attached to the strip.

9. The unitized reagent holder of claim 1, wherein one of the containers holds a release solution.

6. A reagent holder and reagent

system comprising:

a single process tube;

at least three reagent tubes;

a sample lysis reagent in a first said reagent tube;

a PCR reagent in a second said reagent tube;

one or more liquid reagents in a third said reagent tube;

two or more sockets holding pipette tips, wherein the ratio of sockets holding pipette tips to process tubes is at least two; and

wherein the process tube, the two or more sockets configured to hold pipette tips, and the reagent tubes are all joined to a single connecting member.

7. The reagent holder and reagent system of claim 6, further comprising:

a waste tube; and

a pipette tip sheath configured to surround at least one of the pipette tips.

8. The reagent holder and reagent system of claim 6, wherein the connecting member has a first end and a second end, and the process tube is located at either of the first or second ends.