Engineered nucleic acids and methods of use thereof
First Claim
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1. A method of producing in a cell a secreted immunoglobulin having a heavy chain and a light chain, comprising:
- i) providing a target cell capable of protein translation;
ii) introducing into the target cell a composition comprising;
a) a first isolated nucleic acid, wherein the first isolated nucleic acid comprises a first translatable region having at least 95% identity to SEQ ID NO;
5 which encodes the same polypeptide as encoded by SEQ ID NO;
5, andb) a second isolated nucleic acid, wherein the second isolated nucleic acid comprises a second translatable region having at least 95% identity to SEQ ID NO;
7 which encodes the same polypeptide as encoded by SEQ ID NO;
7; and
iii) substantially purifying the secreted immunoglobulin.
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Abstract
Provided are compositions and methods for delivering biological moieties such as modified nucleic acids into cells to modulate protein expression. Such compositions and methods include the use of modified messenger RNAs, and are useful for production of proteins.
947 Citations
16 Claims
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1. A method of producing in a cell a secreted immunoglobulin having a heavy chain and a light chain, comprising:
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i) providing a target cell capable of protein translation; ii) introducing into the target cell a composition comprising; a) a first isolated nucleic acid, wherein the first isolated nucleic acid comprises a first translatable region having at least 95% identity to SEQ ID NO;
5 which encodes the same polypeptide as encoded by SEQ ID NO;
5, andb) a second isolated nucleic acid, wherein the second isolated nucleic acid comprises a second translatable region having at least 95% identity to SEQ ID NO;
7 which encodes the same polypeptide as encoded by SEQ ID NO;
7; andiii) substantially purifying the secreted immunoglobulin. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
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Specification