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Engineered nucleic acids and methods of use thereof

  • US 9,701,965 B2
  • Filed: 11/07/2014
  • Issued: 07/11/2017
  • Est. Priority Date: 10/01/2010
  • Status: Active Grant
First Claim
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1. A method of producing in a cell a secreted immunoglobulin having a heavy chain and a light chain, comprising:

  • i) providing a target cell capable of protein translation;

    ii) introducing into the target cell a composition comprising;

    a) a first isolated nucleic acid, wherein the first isolated nucleic acid comprises a first translatable region having at least 95% identity to SEQ ID NO;

    5 which encodes the same polypeptide as encoded by SEQ ID NO;

    5, andb) a second isolated nucleic acid, wherein the second isolated nucleic acid comprises a second translatable region having at least 95% identity to SEQ ID NO;

    7 which encodes the same polypeptide as encoded by SEQ ID NO;

    7; and

    iii) substantially purifying the secreted immunoglobulin.

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