Aptamers to PDGF and VEGF and their use in treating PDGF and VEGF mediated conditions
First Claim
Patent Images
1. An aptamer comprising the sequence:
- 5′
-GZZQAAEZECZZEZDRGAZZZAAAZGG-3′
(SEQ ID NO.
513)wherein;
each Z is a modified pyrimidine;
Q is selected from any modified or unmodified nucleotide and a substituted or unsubstituted C2-C50 linker, or is absent;
each E is independently selected from a G and a substituted or unsubstituted C2-C50 linker;
D is selected from A and a substituted or unsubstituted C2-C50 linker; and
R is selected from any modified or unmodified nucleotide and a substituted or unsubstituted C2-C50 linker.
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Accused Products
Abstract
Aptamers that bind PDGF and aptamers that bind VEGF are provided. In addition, aptamer constructs comprising a PDGF aptamer and a VEGF aptamer are provided. Pharmaceutical compositions comprising the aptamers and aptamer constructs are provided, as well as methods of treating conditions using the aptamers and aptamer constructs.
49 Citations
10 Claims
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1. An aptamer comprising the sequence:
-
5′
-GZZQAAEZECZZEZDRGAZZZAAAZGG-3′
(SEQ ID NO.
513)wherein; each Z is a modified pyrimidine; Q is selected from any modified or unmodified nucleotide and a substituted or unsubstituted C2-C50 linker, or is absent; each E is independently selected from a G and a substituted or unsubstituted C2-C50 linker; D is selected from A and a substituted or unsubstituted C2-C50 linker; and R is selected from any modified or unmodified nucleotide and a substituted or unsubstituted C2-C50 linker. - View Dependent Claims (2, 3, 4, 5, 9, 10)
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6. The aptamer of 1 further comprising at least one, at least two, at least three, at least four, or at least five nucleosides comprise a 2′
- -OMe.
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7. The aptamer of 1 further comprising at least one, at least two, at least three, at least four, or at least five internucleoside linkages are phosphorothioate linkages.
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8. The aptamer of 1, wherein the aptamer binds VEGF-121 with an affinity of less than 10 nM, less than 5 nM, less than 2 nM, or less than 1 nM.
Specification