Multiplex methylation-specific amplification systems and methods
First Claim
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1. A method, comprising:
- a) treating a specific target nucleic acid with a methylation-specific detection reagent, wherein the specific target nucleic acid is a single gene or untranslated region of a single gene;
b) simultaneously amplifying a plurality of different nucleic acid segments within said specific target nucleic acid to generate a plurality of different amplicons wherein said simultaneous amplifying is carried out in a single reaction vessel; and
c) detecting two or more amplicons of said plurality of different amplicons comprising said different nucleic acids segments within said specific target nucleic acid wherein said detecting comprises contacting said plurality of different amplicons comprising said different nucleic acids segments within said specific target nucleic acid with a plurality of different nucleic acid probes wherein said plurality of different nucleic acid probes comprise a detectable label that is the same label in each of said plurality of different nucleic acid probes.
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Abstract
The present invention relates to systems and methods for performing multiplex amplification reactions. In particular, the present invention relates to multiplex methylation-specific amplification systems and methods.
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11 Claims
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1. A method, comprising:
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a) treating a specific target nucleic acid with a methylation-specific detection reagent, wherein the specific target nucleic acid is a single gene or untranslated region of a single gene; b) simultaneously amplifying a plurality of different nucleic acid segments within said specific target nucleic acid to generate a plurality of different amplicons wherein said simultaneous amplifying is carried out in a single reaction vessel; and c) detecting two or more amplicons of said plurality of different amplicons comprising said different nucleic acids segments within said specific target nucleic acid wherein said detecting comprises contacting said plurality of different amplicons comprising said different nucleic acids segments within said specific target nucleic acid with a plurality of different nucleic acid probes wherein said plurality of different nucleic acid probes comprise a detectable label that is the same label in each of said plurality of different nucleic acid probes. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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11. A method, comprising:
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a) treating a specific target nucleic acid with a methylation-specific detection reagent; b) simultaneously amplifying a plurality of different nucleic acid segments within said specific target nucleic acid to generate a plurality of different amplicons comprising said different nucleic acids segments within said specific target nucleic acid wherein said simultaneous amplifying is carried out in a single reaction vessel; and c) contacting said amplicons with a plurality of different nucleic acid probes to detect said plurality of amplicons comprising said different nucleic acids segments within said specific target nucleic acid wherein said plurality of different nucleic acid probes comprise a detectable label that is the same label in each of said plurality of different nucleic acid probes.
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Specification