Systems, methods, and workflows for optogenetics analysis
First Claim
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1. A method for evaluating the function of a neuron, the method comprising:
- providing a first neuron that includes an optical reporter of electrical activity and a second neuron that is in communication with the first neuron via at least one synapse;
obtaining a signal from the optical reporter in response to a stimulation of the second neuron; and
evaluating the signal in order to determine a functional property of the neuron.
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Abstract
The invention provides methods for characterizing cellular physiology by incorporating into an electrically excitable cell an optical reporter of, and an optical actuator of, electrical activity. A signal is obtained from the optical reporter in response to a stimulation of the cell. Either or both of the optical reporter and actuator may be based on genetically-encoded rhodopsins incorporated into the cell. The invention provides all optical methods that may be used instead of, or as a complement to, traditional patch clamp technologies and that can provide rapid, accurate, and flexible assays of cellular physiology.
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Citations
20 Claims
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1. A method for evaluating the function of a neuron, the method comprising:
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providing a first neuron that includes an optical reporter of electrical activity and a second neuron that is in communication with the first neuron via at least one synapse; obtaining a signal from the optical reporter in response to a stimulation of the second neuron; and evaluating the signal in order to determine a functional property of the neuron. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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11. A method for evaluating the function of a cardiomyocyte, the method comprising:
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providing a first cardiomyocyte that includes an optical reporter of electrical activity and a second cardiomyocyte that is in communication with the first cardiomyocyte via at least one gap junction; obtaining a signal from the optical reporter in response to a stimulation of the second cardiomyocyte; and evaluating the signal in order to determine a functional property of the cardiomyocyte. - View Dependent Claims (12, 13, 14, 15, 16, 17, 18, 19, 20)
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Specification