Device, system and method for processing a sample

• US 9,707,556 B2
• Filed: 08/18/2008
• Issued: 07/18/2017
• Est. Priority Date: 08/17/2007
• Status: Active Grant

• Current Assignee: Diagnostics for the Real World, Ltd.
• Original Assignee: Diagnostics for the Real World, Ltd.
• Inventors: Wisniewski, Craig
• Primary Examiner(s): Xu, Robert
• Application Number:
  US12/673,939
• Publication Number:
  US 20110143339A1
• Time in Patent Office: 3,256 Days
• Field of Search: None
• CPC Class Codes: B01L 2200/0642 Filling fluids into wells by specific techniques
• CPC Class Codes: B01L 2200/10 Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
• CPC Class Codes: B01L 2200/12 Specific details about manufacturing devices
• CPC Class Codes: B01L 2200/16 Reagents, handling or storing thereof
• CPC Class Codes: B01L 2300/049 Valves integrated in closure
• CPC Class Codes: B01L 2300/0636 Integrated biosensor, microarrays
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0825 Test strips
• CPC Class Codes: B01L 2300/087 Multiple sequential chambers
• CPC Class Codes: B01L 2300/0887 Laminated structure
• CPC Class Codes: B01L 2300/123 Flexible; Elastomeric
• CPC Class Codes: B01L 2300/1827 using resistive heater
• CPC Class Codes: B01L 2400/0481 squeezing of channels or chambers
• CPC Class Codes: B01L 2400/049 vacuum
• CPC Class Codes: B01L 2400/0638 membrane valves, flap valves
• CPC Class Codes: B01L 3/5023 with a sample being transported to, and subsequently stored in an absorbent for analysis
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: B01L 9/527 for microfluidic devices, e.g. used for lab-on-a-chip
• CPC Class Codes: G01N 2035/00366 Several different temperatures used
• CPC Class Codes: G01N 2035/00534 Mixing by a special element, e.g. stirrer

Abstract

A device for processing a sample comprises a blister defined by first and second walls. The first wall is flexible allowing the blister to be divided into one or more sealed regions by an external pressure applied to a portion of the first wall. The external pressure is applied in the form of a 2-dimensional shape to form a sealed region having that shape.

Claims

1. A device for the processing of a sample comprising,

a single blister only, the blister defined between first and second walls wherein the first wall is flexible such that the blister can be divided into one or more sealed chambers by an external pressure applied to a portion of the first wall that urges the first wall towards the second wall, the pressure being applied in the form of a two dimensional shape to form a sealed chamber in that shape, wherein the blister, once divided by operation of the external pressure, is operable to become undivided when the external pressure is released.

2. A device according to claim 1 in which the first wall is flexible such that a localised external pressure urging a portion of the first wall towards the second wall can produce a localised seal.

3. A device according to claim 1 in which an object is retained at a location within the blister.

4. A device according to claim 3 in which the object comprises a dried reagent or a sachet of liquid.

5. A device according to claim 1 in which the first wall is shaped, or has a plurality of dimples, for locating objects at predetermined locations within the blister.

6. A device according to claim 1 in which the second wall has recesses for locating objects.

7. A device according to claim 1, further comprising a sample introduction port to allow introduction of the sample into the blister.

8. A device according to claim 1, further comprising a vacuum port to allow removal of air or a gas from the blister.

9. A device according to claim 1, further comprising a gasket disposed within the blister, in which localised pressure urging the first wall towards the second wall with the gasket in-between can form a localised seal.

10. A device according to claim 9 in which the gasket comprises a material that is disposed on the second wall within the blister to define one or more sealable regions in the blister, the sealable regions being sealable on application of a suitable external force or pressure.

11. A device according to claim 1 in which the blister can be divided into one or more sealed regions by application of one or more shaped platens that urge the first wall towards the second wall.

12. A device according to claim 11 in which the sealed regions can be sealed and unsealed sequentially to allow the sample and/or reagents to pass between the regions in a predetermined manner.

13. A device according to claim 11 in which one or more of the sealed regions contains a processing reagent or processing reagents and removal of the seals allows the sample to contact the processing reagent or reagents.

14. A device according to claim 1 in which the sample may be mixed by an oscillating external pressure applied to at least a portion of the blister.

15. A device according to claim 1, further comprising an analysis means.

16. A device according to claim 15 in which the analysis means is located within a sealable region of the blister.

17. A device according to claim 15 in which the analysis means is located in an analysis chamber in communication with the blister.

18. A device according to claim 17 in which the second wall has an exit port to allow passage of a processed sample from the blister to the analysis chamber.

19. A device according to claim 15 in which the analysis means comprises a test strip.

20. A device according claim 1 in which the second wall is a base plate and the first wall is a flexible film disposed on the base plate.

21. A device according to claim 1 for the processing of a biological, chemical or environmental sample.

22. A device according to claim 1 for the amplification and detection of nucleic acid or genetic material in a biological or environmental sample.

23. A device according to claim 1 in which the blister is sealed from the external environment.

24. A device for the processing of a sample comprising, it single blister only, the blister defining a single cavity and being defined between first and second walls, wherein the first wall is flexible such that the blister can be divided into one or more sealed chambers by an external pressure applied to a portion of the first wall that urges the first wall towards the second wall, the pressure being applied in the form of a two dimensional shape to form a sealed chamber in that shape, wherein the blister, once divided by operation of the external pressure, is operable to become undivided when the external pressure is released.