×

System for capturing and analyzing cells

  • US 9,707,562 B2
  • Filed: 03/18/2016
  • Issued: 07/18/2017
  • Est. Priority Date: 03/13/2013
  • Status: Active Grant
First Claim
Patent Images

1. A system for processing a biological sample, comprising:

  • a reservoir, including a reservoir inlet and a reservoir outlet, configured to receive at least one of the biological sample and a process fluid;

    a fluid delivery module including a cylindrical cartridge having a set of chambers that hold the at least one fluid for processing the biological sample and an actuator coupled to a piercer configured to access at least one chamber of the set of chambers, wherein the cylindrical cartridge is rotatable about an axis to align a chamber of the set of chambers with the piercer, to facilitate flow of contents of the chamber into the reservoir inlet, and wherein the set of chambers is distributed about and offset from the axis;

    a manifold configured to receive and deliver at least one of the biological sample and the process fluid from the reservoir to a biological sample substrate, the manifold comprising;

    a broad surface comprising a central region configured to receive the biological sample substrate during operation of the system; and

    a set of openings defined at the central region, the set of openings comprising;

    a first subset of openings configured to transmit fluid flow to the biological sample substrate; and

    a second subset of openings configured to receive the fluid flow from the biological sample substrate, wherein the first and the second subsets of openings are symmetrically opposed across a manifold axis;

    a manifold inlet configured at an upstream end of the broad surface, the manifold inlet coupled to the reservoir and to the first subset of openings, and the manifold inlet configured to transmit flow from the reservoir to the first subset of openings;

    a manifold outlet configured at a downstream end of the broad surface and coupled to the second subset of openings, the manifold outlet configured to transmit waste fluid from the manifold; and

    a magnet proximal the reservoir, wherein in a first configuration, the magnet retains a concentration of contaminating particles from the biological sample when a flow of the biological sample is driven from the reservoir in a forward direction to the manifold inlet.

View all claims
  • 4 Assignments
Timeline View
Assignment View
    ×
    ×