Bacterial culture media and methods for their preparation and use

• US 9,708,580 B2
• Filed: 05/15/2012
• Issued: 07/18/2017
• Est. Priority Date: 05/15/2012
• Status: Expired due to Fees

• Current Assignee: Shanghai Zhiyi Information Technology Limited
• Original Assignee: Shanghai Zhiyi Information Technology Limited
• Inventors: Hong, Feng
• Primary Examiner(s): Knight, Teresa E
• Application Number:
  US13/885,390
• Publication Number:
  US 20140024075A1
• Time in Patent Office: 1,890 Days
• CPC Class Codes: C12N 1/20 Bacteria; Culture media therefor
• CPC Class Codes: C12N 1/22 Processes using, or culture media containing, cellulose or hydrolysates thereof
• CPC Class Codes: C12P 19/04 Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
• CPC Class Codes: C12P 19/14 produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase , e.g. by cellulase, hemicellulase

Abstract

Provided herein are methods and compositions for bacterial cellulose production. In some embodiments, the methods and compositions involve or are made from distiller'"'"'s grain.

Claims

1. A culture medium for bacterial cellulose production mixture, the culture medium comprising:

a detoxified distiller'"'"'s grain hydrolysate;

a carbon source;

wherein the carbon source is present from about 0.1% (w/v) to 20% (w/v) of the detoxified distiller'"'"'s grain hydrolysate;

a nitrogen source;

a salt source;

a bacterium capable of producing bacterial cellulose; and

bacterial cellulose.

2. The bacterial cellulose production mixture of claim 1, wherein the carbon source comprises sugars of hydrolyzed lignocellulose.

3. The bacterial cellulose production mixture of claim 1, wherein the carbon source includes at least one of cellulose or hemicellulose.

4. The bacterial cellulose production mixture of claim 1, wherein the carbon source comprises a sugar.

5. The bacterial cellulose production mixture of claim 1, further comprising at least one of a maize alcohol fermentation filtrate, a maize alcohol fermentation supernatant, a distiller'"'"'s grain filtrate, a distiller'"'"'s grain supernatant, or a distiller'"'"'s grain hydrolysate;

wherein the carbon source is present at about 0.1% (w/v) to about 20% (w/v) of the amount of at least one of the maize alcohol fermentation filtrate, the maize alcohol fermentation supernatant, the distiller'"'"'s grain filtrate, the distiller'"'"'s grain supernatant, or the distiller'"'"'s grain hydrolysate.

6. The bacterial cellulose production mixture of claim 1, wherein the nitrogen source is present at about 0.1% (w/v) to about 2% (w/v) of the amount of the detoxified distiller'"'"'s grain hydrolysate.

7. The bacterial cellulose production mixture of claim 1, further comprising at least one of a maize alcohol fermentation filtrate, a maize alcohol fermentation supernatant, a distiller'"'"'s grain filtrate, a distiller'"'"'s grain supernatant, or a distiller'"'"'s grain hydrolysate.

8. The bacterial cellulose production mixture of claim 1, wherein the at least one bacterium capable of producing bacterial cellulose includes at least one of Acetobacter, Azotobacter, Rhizobium, Pseudomonas, Salmonella, Alcaligenes, Sarcina ventriculi, Agrobacterium, xylinum, A. hansenii, A. pasteurianus, or Gluconacetobacter xylinus.

9. A culture medium for bacterial cellulose production mixture, the culture medium comprising:

a detoxified distiller'"'"'s grain hydrolysate;

a carbon source including a sugar that is present in an amount of about 0.1% (w/v) to about 20% (w/v) the amount of the detoxified distiller'"'"'s grain hydrolysate;

a nitrogen source present at about 0.1% (w/v) to about 2% (w/v) of the amount of the detoxified distiller'"'"'s grain hydrolysate;

a salt source;

a bacterium capable of producing bacterial cellulose; and

bacterial cellulose.

10. The bacterial cellulose production mixture of claim 9, further comprising at least one of a maize alcohol fermentation filtrate, a maize alcohol fermentation supernatant, a distiller'"'"'s grain filtrate, a distiller'"'"'s grain supernatant, or a distiller'"'"'s grain hydrolysate.

11. The bacterial cellulose production mixture of claim 9, wherein the carbon source comprises sugars of hydrolyzed lignocellulose.

12. The bacterial cellulose production mixture of claim 9, wherein the carbon source further includes at least one of cellulose or hemicellulose.

13. The bacterial cellulose production mixture of claim 9, wherein the at least one bacterium capable of producing bacterial cellulose includes at least one of Acetobacter, Azotobacter, Rhizobium, Pseudomonas, Salmonella, Alcaligenes, Sarcina ventriculi, Agrobacterium, xylinum, A. hansenii, A. pasteurianus, or Gluconacetobacter xylinus.

14. A method of producing bacterial cellulose, the method comprising:

inoculating at least one bacterium capable of producing bacterial cellulose in a bacterial cellulose production mixture comprising;

a detoxified distiller'"'"'s grain hydrolysate;

a carbon source;

wherein the carbon source is present from about 0.1% (w/v) to 20% (w/v) of the detoxified distiller'"'"'s grain hydrolysate;

a nitrogen source; and

a salt source

cultivating the at least one bacterium capable of producing bacterial cellulose in the bacterial cellulose production mixture to produce bacterial cellulose; and

harvesting the bacterial cellulose.

15. The method of claim 14, wherein the bacterial cellulose production mixture further comprises a distiller'"'"'s grain hydrolysate.

16. The method of claim 15, wherein the hydrolysate includes at least one of an enzymatic hydrolysate or a detoxified acid hydrolysate.