Modulation of expression of acyltransferases to modify hydroxycinnamic acid content

• US 9,708,624 B2
• Filed: 06/22/2015
• Issued: 07/18/2017
• Est. Priority Date: 12/21/2012
• Status: Active Grant

• Current Assignee: Regents of the University of California (University of California)
• Original Assignee: Regents of the University of California (University of California)
• Inventors: Scheller, Henrik Vibe, Bartley, Laura E., Ronald, Pamela
• Primary Examiner(s): Kallis, Russell
• Application Number:
  US14/746,779
• Publication Number:
  US 20150307892A1
• Time in Patent Office: 757 Days
• Field of Search: None
• CPC Class Codes: C12N 15/8246 Non-starch polysaccharides, e.g. cellulose, fructans, levans
• CPC Class Codes: C12N 9/1025 Acyltransferases (2.3)

Abstract

The invention provides methods of engineering plants to modulate hydroxycinnamic acid content. The invention additionally provides compositions and methods comprising such plants.

Claims

1. A method of engineering a plant to decrease the ferulic acid content in the plant, the method comprising:

introducing an expression cassette into the plant, wherein the expression cassette comprises a polynucleotide encoding an AT10 acyltransferase, and wherein the acyltransferase has at least 95% identity to amino acids 4 to 434 of SEQ ID NO;

2,

culturing the plant under conditions in which the acyltransferase is overexpressed, and

selecting a plant that has a decreased ferulic acid content compared to a wildtype plant in which the acyltransferase is not overexpressed.

2. The method of claim 1, wherein the polynucleotide is operably linked to a promoter endogenous to the plant.

3. The method of claim 1, wherein the expression cassette comprises a promoter to which the polynucleotide is operably linked.

4. The method of claim 3, wherein the promoter is a tissue-specific promoter.

5. The method of claim 4, wherein the promoter drives expression in cell wall.

6. The method of claim 5, wherein the polynucleotide has at least 70% identity to SEQ ID NO:

1.

7. The method of claim 1, wherein the plant is a grass plant.

8. The method of claim 7, wherein the grass plant is rice, corn, switchgrass, sorghum, millet, miscanthus, sugarcane, alfalfa, wheat, soy, rye, barley, turfgrass, hemp, bamboo, rape, sunflower, or brachypodium.

9. A plant engineered by the method of claim 1, or a transgenic progeny of the plant.

10. A plant cell of the plant or the transgenic progeny of the plant of claim 9.

11. Biomass comprising the plant or a part of the plant of claim 9.

12. A method of obtaining an increased amount of soluble sugars from a plant in a saccharification reaction, the method comprising:

subjecting the plant of claim 9 to a saccharification reaction, thereby increasing the amount of soluble sugars that can be obtained from the plant as compared to a wild-type plant.

13. The method of claim 1, wherein the acyltransferase comprises amino acids 4 to 434 of SEQ ID NO:

2.