Compound arrays for sample profiling
First Claim
1. A method of testing a vaccine, comprising(a) contacting antibodies in a blood sample of a subject immunized with a vaccine against a pathogenic microorganism with an array of different peptides of between 10-30 amino acids in length immobilized on the surface of the array, wherein each different peptide has a unique amino acid sequence, each of the different peptides occupying a distinct feature of the array, wherein each distinct feature of the array comprises peptides of a same sequence, wherein at least 50 of the different peptides have dissociation constants of between 1 mM and 1 μ
- M to at least one antibody in the subject'"'"'s sample, and wherein the array comprises at least 100,000 immobilized different peptides;
(b) detecting a pattern of antibody binding of the blood sample to the immobilized different peptides in the array, wherein the pattern comprises binding interactions with dissociation constants of between 1 mM and 1 μ
M; and
(c) comparing the binding interactions with dissociation constants of between 1 mM and 1 μ
M in the pattern of antibody binding of the blood sample of the subject to the pattern of antibody binding of one or more reference samples, wherein the reference samples are from subjects who have survived an infection with the pathogenic microorganism, wherein a plurality of peptides common to the pattern of antibody binding of the subject and pattern of antibody binding of the one or more reference samples indicates that the vaccine is effective against the pathogenic microorganism.
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Abstract
The invention provides arrays of compound for use in profiling samples. The arrays include compounds bind to components of the samples at relatively low affinities. The avidity of compounds binding to components of the samples can be increased by forming arrays such that multivalent components of the samples (e.g., antibodies or cells) can bind to more than one molecule of a compound at the same time. When a sample is applied to an array under such conditions, the compounds of the array bind to component(s) of the sample with significantly different avidities generating a profile characteristic of the sample.
96 Citations
23 Claims
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1. A method of testing a vaccine, comprising
(a) contacting antibodies in a blood sample of a subject immunized with a vaccine against a pathogenic microorganism with an array of different peptides of between 10-30 amino acids in length immobilized on the surface of the array, wherein each different peptide has a unique amino acid sequence, each of the different peptides occupying a distinct feature of the array, wherein each distinct feature of the array comprises peptides of a same sequence, wherein at least 50 of the different peptides have dissociation constants of between 1 mM and 1 μ - M to at least one antibody in the subject'"'"'s sample, and wherein the array comprises at least 100,000 immobilized different peptides;
(b) detecting a pattern of antibody binding of the blood sample to the immobilized different peptides in the array, wherein the pattern comprises binding interactions with dissociation constants of between 1 mM and 1 μ
M; and(c) comparing the binding interactions with dissociation constants of between 1 mM and 1 μ
M in the pattern of antibody binding of the blood sample of the subject to the pattern of antibody binding of one or more reference samples, wherein the reference samples are from subjects who have survived an infection with the pathogenic microorganism, wherein a plurality of peptides common to the pattern of antibody binding of the subject and pattern of antibody binding of the one or more reference samples indicates that the vaccine is effective against the pathogenic microorganism. - View Dependent Claims (2, 3, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23)
- M to at least one antibody in the subject'"'"'s sample, and wherein the array comprises at least 100,000 immobilized different peptides;
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4. The method of 1, wherein the peptides are 12 to 35 amino acids long.
Specification