Biosensors and biosensing incorporating RF and microwave radiation

• US 9,709,560 B2
• Filed: 09/29/2008
• Issued: 07/18/2017
• Est. Priority Date: 09/29/2008
• Status: Active Grant

• Current Assignee: Intel Corporation
• Original Assignee: Intel Corporation
• Inventors: Horesh, Moran
• Primary Examiner(s): Shibuya, Mark
• Assistant Examiner(s): Moerschell, Richard
• Application Number:
  US12/286,383
• Publication Number:
  US 20100081132A1
• Time in Patent Office: 3,214 Days
• Field of Search: 422 50
• CPC Class Codes: C12Q 2565/607 being a sensor, e.g. electrode
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• CPC Class Codes: G01N 33/5438 Electrodes
• CPC Class Codes: H01L 29/78 with field effect produced by an insulated gate H01L29/7725, H01L29/775, H01L29/778 take precedence
• CPC Class Codes: Y10S 435/817 Enzyme or microbe electrode

Abstract

Devices capable applying microwave and RF electromagnetic radiation to specific molecular interactions between a capture molecule and a target analyte and capable of sensing the specific molecular interaction are provided. Specific molecular interactions include, for example, specific recognition events such as, receptor-ligand, antigen-antibody, DNA-protein, sugar-lectin, RNA-ribosome, and enzyme substrate interactions and nucleic acid-nucleic acid hybridizations. Additionally, methods are provided for detecting the presence or absence of a target analyte in a sample. The presence or absence of the target analyte is detected, in part, through the detection of a binding complex between the target analyte and a capture molecule in the presence of microwave and or RF electromagnetic radiation.

Claims

1. A device capable of sensing a biological interaction, wherein the device comprises

a substrate having a surface and a field effect transistor wherein the field effect transistor has a sensing surface and the sensing surface is in the substrate surface and wherein the field effect transistor is capable of sensing the occurrence of biological events at the sensing surface and wherein the biological events are an occurrence of an enzymatic catalytic reaction,

a first module capable of generating electromagnetic radiation having a frequency in the microwave or radiofrequency range housed in the substrate, wherein the first module comprises a transmitting element connected to a circuit to cause the production of microwave or radiofrequency radiation by the transmitting element, and

a second module for causing the first module capable of generating electromagnetic radiation to irradiate the sensing surface with a known frequency of microwave or radiofrequency electromagnetic radiation wherein the known frequency of microwave or radiofrequency electromagnetic radiation is selected to enhance a signal obtained by the field effect transistor for a molecule to be detected relative to molecules that are not to be detected;

and

an enzyme on the sensing surface capable of catalyzing a chemical reaction in the presence of a target analyte.

2. The device of claim 1 wherein the device additionally comprises capture molecules capable of specifically recognizing the analyte that are located on the sensing surface.

3. The device of claim 1 wherein the device additionally comprises capture molecules capable of specifically recognizing the analyte located on the sensing surface and the capture molecule is molecules are selected from the group consisting of nucleic acids, peptides, and antibodies.

4. The device of claim 1 wherein the first module capable of generating electromagnetic radiation having a frequency in the microwave or radiofrequency range is capable of generating radiation having a frequency from 1 GHz to 50 GHz.

5. The device of claim 1 wherein the device additionally comprises a plurality of field effect transistors that form an array.

6. The device of claim 5 wherein the plurality of field effect transistors additionally comprises capture molecules capable of specifically recognizing the analyte.

7. The device of claim 1 wherein the first module capable of generating microwave or radiofrequency radiation is capable of generating radiation that has a maximum transmission range of 2.5 mm.

21. The device of claim 1 wherein the first module is configured to scan electromagnetic radiation over a frequency range included within a range of 3 Hz-300 GHz.

8. A device capable of sensing a biological interaction, wherein the device comprises

a substrate having a surface and a sensor wherein the sensor has a sensing element that is comprised of an electrode having a surface and the electrode is in the substrate surface and wherein the sensor is capable of sensing the occurrence of biological events at the electrode surface and wherein the electrode surface comprises capture molecules capable of specifically recognizing a target analyte,

a first module capable of generating electromagnetic radiation having a frequency in the microwave or radiofrequency range housed in the substrate, wherein the first module comprises a transmitting element connected to a circuit to cause the production of microwave or radiofrequency radiation by the transmitting element, and

a second module for causing the first module capable of generating electromagnetic radiation to irradiate the electrode surface with a known frequency of microwave or radiofrequency electromagnetic radiation wherein the known frequency of microwave or radiofrequency electromagnetic radiation is selected to enhance a signal obtained by the sensing element for a molecule to be detected relative to molecules that are not to be detected;

and

an enzyme on the electrode surface capable of catalyzing a chemical reaction in the presence of the target analyte.

9. The device of claim 8 wherein at least one of the capture molecules is selected from the group consisting of nucleic acids, peptides, and antibodies.

10. The device of claim 8 wherein the first module capable of generating electromagnetic radiation having a frequency in the microwave or radiofrequency range is capable of generating radiation having a frequency from 1 GHz to 50 GHz.

11. The device of claim 8 wherein the device additionally comprises a plurality of sensors that form an array of sensors.

12. The device of claim 11 wherein the plurality of sensors additionally comprises capture molecules capable of specifically recognizing the target analyte.

13. The device of claim 8 wherein the first module capable of generating microwave or radiofrequency radiation is capable of generating radiation that has a maximum transmission range of 2.5 mm.

22. The device of claim 8 wherein the first module is configured to scan electromagnetic radiation over a frequency range included within a range of 3 Hz-300 GHz.

14. A device capable of sensing a biological interaction, wherein the device comprises:

a substrate;

a field effect transistor (FET), which includes a sensing surface, on the substrate;

wherein;

(a)(i) the FET is capable of sensing the occurrence of biological events at the sensing surface, and (a)(ii) the biological events are an occurrence of an enzymatic catalytic reaction;

a first module on the substrate and including at least one transmitting element;

wherein the first module and the at least one transmitting element are configured to scan electromagnetic radiation over a frequency range included within a range of 3 Hz-300 GHz;

a second module to cause the first module to scan the radiation over the frequency range and onto the sensing surface;

wherein the frequency range is configured to enhance a signal obtained by the FET for a molecule to be detected relative to molecules that are not to be detected, and

an enzyme on the sensing surface capable of catalyzing a chemical reaction in the presence of a target analyte.

15. The device of claim 14 comprising an array of FETs on the substrate, wherein each of the FETs has a sensing surface.

16. The device of claim 15 wherein the at least one transmitting element substantially surrounds the array in a plane.

17. The device of claim 16 wherein at least one of the at least one transmitting element is underneath the sensing surface and between the sensing surface and the substrate.

18. The device of claim 16 wherein at least one of the at least one transmitting element includes metal lines.

19. The device of claim 16 further comprising an electrode, separate from the sensing surface, which is capable of applying a voltage between an electrolyte on the sensing surface and a source of the FET.

20. The device of claim 19 comprising an insulation layer on the sensing surface.