Production of fatty alcohols from engineered microorganisms

• US 9,714,437 B2
• Filed: 05/09/2016
• Issued: 07/25/2017
• Est. Priority Date: 04/20/2012
• Status: Active Grant

• Current Assignee: Codexis, Inc.
• Original Assignee: Codexis, Inc.
• Inventors: Krebber, Claus M, Mitchell, Kenneth, Chan, Kaman, Valle, Fernando, Barak, Yoram, Clark, Louis, Karlshoej, Kristian, Vroom, Jonathan, Nazor, Jovana
• Primary Examiner(s): Monshipouri, Maryam
• Application Number:
  US15/149,859
• Publication Number:
  US 20160244787A1
• Time in Patent Office: 442 Days
• CPC Class Codes: A61K 2800/10 General cosmetic use
• CPC Class Codes: A61K 8/342 Alcohols having more than seven atoms in an unbroken chain
• CPC Class Codes: A61Q 19/00 Preparations for care of the skin
• CPC Class Codes: C07C 31/125 containing five to twenty-two carbon atoms
• CPC Class Codes: C11D 3/2031 fatty or with at least 8 carbon atoms in the alkenyl chain
• CPC Class Codes: C12N 15/70 Vectors or expression systems specially adapted for E. coli
• CPC Class Codes: C12N 9/0008 acting on the aldehyde or oxo group of donors (1.2)
• CPC Class Codes: C12N 9/1029 transferring groups other than amino-acyl groups (2.3.1)
• CPC Class Codes: C12N 9/16 acting on ester bonds (3.1)
• CPC Class Codes: C12N 9/93 Ligases (6)
• CPC Class Codes: C12P 5/02 acyclic C12P5/007 takes precedence
• CPC Class Codes: C12P 7/04 acyclic
• CPC Class Codes: C12P 7/6436 Fatty acid esters
• CPC Class Codes: C12Y 102/0105 Long-chain-fatty-acyl-CoA reductase (1.2.1.50)
• CPC Class Codes: C12Y 203/01086 Fatty-acyl-CoA synthase (2.3.1.86)
• CPC Class Codes: C12Y 301/02 Thioester hydrolases (3.1.2)
• CPC Class Codes: C12Y 301/02014 Oleoyl-[acyl-carrier-protein] hydrolase (3.1.2.14), i.e. ACP-thioesterase
• CPC Class Codes: C12Y 602/01003 Long-chain-fatty-acid-CoA ligase (6.2.1.3)
• CPC Class Codes: Y02E 50/10 Biofuels, e.g. bio-diesel

Abstract

The invention generally relates to the production of a fatty alcohol composition from recombinant microbial cells. The fatty alcohols are produced by expressing a gene encoding a heterologous fatty alcohol forming acyl-CoA reductase (“FAR”); a gene encoding a heterologous thioesterase (“TE”) gene and a gene encoding an acyl-CoA synthetase (“ACS”).

Claims

1. An expression vector comprising the recombinant polynucleotide sequence of SEQ ID NO:

16.

2. The expression vector of claim 1, wherein said vector further comprises a gene of interest.

5. A host cell comprising the expression vector of claim 1.

3. An expression vector comprising the recombinant polynucleotide sequence of SEQ ID NO:

62.

4. The expression vector of claim 3, wherein said vector further comprises a gene of interest.

6. A host cell comprising the expression vector of claim 3.

7. A recombinant polynucleotide sequence comprising SEQ ID NO:

16.

8. A recombinant polynucleotide sequence comprising SEQ ID NO:

62.