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Mediator-stabilized reagent compositions for use in biosensor electrodes

  • US 9,714,441 B2
  • Filed: 04/08/2014
  • Issued: 07/25/2017
  • Est. Priority Date: 10/12/2007
  • Status: Active Grant
First Claim
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1. A method of determining concentration of an analyte, the method comprising:

  • contacting a sample with a biosensor strip comprising;

    an electrode support;

    a working electrode disposed on the electrode support;

    a reagent composition deposited on the working electrode, wherein the reagent composition comprises;

    an enzyme, anda redox mediator complex comprising;

    a first metal ion covalently bonded to at least one compound comprising a 1,2-quinine moiety; and

    Li+, Be2+, Sr2+, Sc3+, Y3+, La3+, Ce3+, Pr3+, Nd3+, Pm3+, Sm3+, Eu3+, Gd3+, Tb3+, Dy3+, Ho3+, Er3+, Tm3+, Yb3+, or Lu3+ions non-covalently bonded to the oxygen atoms of the 1,2-quinone moiety,wherein the first metal ion and the second metal ion are different;

    applying a potential to the working electrode;

    measuring a current from the working electrode; and

    determining the analyte concentration.

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