Mediator-stabilized reagent compositions for use in biosensor electrodes
First Claim
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1. A method of determining concentration of an analyte, the method comprising:
- contacting a sample with a biosensor strip comprising;
an electrode support;
a working electrode disposed on the electrode support;
a reagent composition deposited on the working electrode, wherein the reagent composition comprises;
an enzyme, anda redox mediator complex comprising;
a first metal ion covalently bonded to at least one compound comprising a 1,2-quinine moiety; and
Li+, Be2+, Sr2+, Sc3+, Y3+, La3+, Ce3+, Pr3+, Nd3+, Pm3+, Sm3+, Eu3+, Gd3+, Tb3+, Dy3+, Ho3+, Er3+, Tm3+, Yb3+, or Lu3+ions non-covalently bonded to the oxygen atoms of the 1,2-quinone moiety,wherein the first metal ion and the second metal ion are different;
applying a potential to the working electrode;
measuring a current from the working electrode; and
determining the analyte concentration.
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Abstract
The claimed subject matter relates to the stabilization of 1,2-quinone mediators, especially those containing 1,10-phenanthroline quinone (PQ) and more especially transition metal complexes of PQ, in the presence of enzymes when contained in dry reagent layers for biosensor electrodes, through the use of various metal salts, particularly those of lithium.
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9 Claims
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1. A method of determining concentration of an analyte, the method comprising:
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contacting a sample with a biosensor strip comprising;
an electrode support;a working electrode disposed on the electrode support; a reagent composition deposited on the working electrode, wherein the reagent composition comprises; an enzyme, and a redox mediator complex comprising; a first metal ion covalently bonded to at least one compound comprising a 1,2-quinine moiety; and Li+, Be2+, Sr2+, Sc3+, Y3+, La3+, Ce3+, Pr3+, Nd3+, Pm3+, Sm3+, Eu3+, Gd3+, Tb3+, Dy3+, Ho3+, Er3+, Tm3+, Yb3+, or Lu3+ions non-covalently bonded to the oxygen atoms of the 1,2-quinone moiety, wherein the first metal ion and the second metal ion are different; applying a potential to the working electrode; measuring a current from the working electrode; and determining the analyte concentration. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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Specification