Transgenic plant event detection
First Claim
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1. A kit for examining a sample for the presence or absence of material derived from one or more transgenic plant events, the kit comprising primer pairs for amplification of one, more than one or all of nucleic acids chosen from:
- a) “
Zm”
;
a nucleic acid derived from and specific for Zea mays taxon,b) “
Bn”
;
a nucleic acid derived from and specific for Brassica napus taxon,c) “
Gm”
;
a nucleic acid derived from and specific for Glycine max taxon,o) “
Or”
;
a nucleic acid derived from and specific for Oryza sativa taxon,p) “
Bv”
;
a nucleic acid derived from and specific for Beta vulgaris taxon,q) “
Gs”
;
a nucleic acid derived from and specific for Gossypium taxon, andt) “
St”
;
a nucleic acid derived from and specific for Solanum tuberosum taxon; and
primer pairs for amplification of all of nucleic acids d)-i);
d) “
p35S”
;
a nucleic acid derived from the 35S promoter of Cauliflower Mosaic Virus,e) “
tNOS”
;
a nucleic acid derived from the 3′
terminator of the Agrobacterium tumefaciens nopaline synthetase gene,f) “
Cry1Ab”
;
a nucleic acid derived from the crystal protein gene Cry1Ab of Bacillus thuringiensis, g) “
PAT/bar”
;
a nucleic acid derived from the phosphinothricin acetyltransferase (PAT) gene bar of Streptomyces hygroscopicus, h) “
PAT/pat”
;
a nucleic acid derived from the phosphinothricin acetyltransferase (PAT) gene pat of Streptomyces viridochromogenes, andi) “
CP4-EPSPS”
;
a nucleic acid derived from the 5-Enol-pyruvylshikimate-3-phosphate synthase EPSPS gene from Agrobacterium sp. CP4;
wherein the respective primer pairs are from the following table, or variants showing at least 85% sequence identity to said primer pairs;
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Abstract
The present invention relates to detection of materials derived from transgenic plant events. In particular, the invention provides methods, reagents, kits and reference materials for detecting the presence or absence in a sample of genetic material derived from and attributable to select transgenic plant events.
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Citations
9 Claims
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1. A kit for examining a sample for the presence or absence of material derived from one or more transgenic plant events, the kit comprising primer pairs for amplification of one, more than one or all of nucleic acids chosen from:
-
a) “
Zm”
;
a nucleic acid derived from and specific for Zea mays taxon,b) “
Bn”
;
a nucleic acid derived from and specific for Brassica napus taxon,c) “
Gm”
;
a nucleic acid derived from and specific for Glycine max taxon,o) “
Or”
;
a nucleic acid derived from and specific for Oryza sativa taxon,p) “
Bv”
;
a nucleic acid derived from and specific for Beta vulgaris taxon,q) “
Gs”
;
a nucleic acid derived from and specific for Gossypium taxon, andt) “
St”
;
a nucleic acid derived from and specific for Solanum tuberosum taxon; andprimer pairs for amplification of all of nucleic acids d)-i); d) “
p35S”
;
a nucleic acid derived from the 35S promoter of Cauliflower Mosaic Virus,e) “
tNOS”
;
a nucleic acid derived from the 3′
terminator of the Agrobacterium tumefaciens nopaline synthetase gene,f) “
Cry1Ab”
;
a nucleic acid derived from the crystal protein gene Cry1Ab of Bacillus thuringiensis,g) “
PAT/bar”
;
a nucleic acid derived from the phosphinothricin acetyltransferase (PAT) gene bar of Streptomyces hygroscopicus,h) “
PAT/pat”
;
a nucleic acid derived from the phosphinothricin acetyltransferase (PAT) gene pat of Streptomyces viridochromogenes, andi) “
CP4-EPSPS”
;
a nucleic acid derived from the 5-Enol-pyruvylshikimate-3-phosphate synthase EPSPS gene from Agrobacterium sp. CP4;wherein the respective primer pairs are from the following table, or variants showing at least 85% sequence identity to said primer pairs; - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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Specification
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Current AssigneeSciensano Co.
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Original AssigneeScientific Institute of Public Health
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InventorsMbongolo Mbella, Etondoh Guillaume, Van Den Bulcke, Marc Henri Germain, Lievens, Antoon Piet Nelly Raoul, Leunda, Amaya, Barbau-Piednoir, Elodie, Sneyers, Myriam Jacqueline Sylviane
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Primary Examiner(s)SIMS, JASON M
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Application NumberUS14/174,941Publication NumberTime in Patent Office1,264 DaysField of SearchNoneUS Class CurrentCPC Class CodesC12Q 1/6844 Nucleic acid amplification ...C12Q 1/6895 for plants, fungi or algaeC12Q 2537/143 Multiplexing, i.e. use of m...C12Q 2600/16 Primer sets for multiplex a...Y02A 40/146 Genetically Modified [GMO] ...
