Compositions for fabric based lateral flow assay device using electrochemical detection means, and devices therefrom

• US 9,714,912 B2
• Filed: 10/07/2012
• Issued: 07/25/2017
• Est. Priority Date: 08/13/2012
• Status: Active Grant

• Current Assignee: Achira Labs Pvt. Ltd.
• Original Assignee: Achira Labs Pvt. Ltd.
• Inventors: Lal, Ashish Kumar, Choudhary, Tripurari, Gururaj, K B, Tahasildar, Manjunath
• Primary Examiner(s): DINH, BACH T
• Application Number:
  US14/421,406
• Publication Number:
  US 20150241379A1
• Time in Patent Office: 1,752 Days
• Field of Search: 20440301-40315
• CPC Class Codes: C12Q 1/002 Electrode membranes
• CPC Class Codes: G01N 27/3275 Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
• CPC Class Codes: G01N 33/5438 Electrodes
• CPC Class Codes: G01N 33/558 using diffusion or migration of antigen or antibody immunochromatographic test strips G01N33/54387

Abstract

The invention provides a composition for lateral flow assay device that comprises at least one fabric strand coated with a conducting material and a diagnostic material. The invention also provides a lateral flow assay device(10) having a proximal end(12), a distal end(14) and a testing zone intermediate between the proximal end(12) and the distal end(14). The lateral flow assay device(20) is made of a fabric comprising at least one strand(22) that defines a flow path. The at least one strand of the fabric in the testing zone is coated with a conducting material(24). The at least one strand is made of at least one of a cotton, a silk-based material, and combinations thereof. The invention also provides a lateral flow assay system(40) comprising the lateral flow assay device(42) of the invention, wherein the at least one electrode(52) connected to the strand(50) coated with the conducting material and measurement means(54) to measure the output from the at least one electrode(52).

Claims

1. An electrochemical assay device comprising a fabric made of a plurality of strands interwoven together, wherein the fabric has a proximal end, a distal end and a testing zone intermediate between the proximal end and the distal end, and wherein the plurality of strands comprises:

at least one conducting strand comprising a conducting material at least in the testing zone;

at least one hydrophilic strand; and

at least one hydrophobic strand, wherein the at least one hydrophobic strand is interwoven with the at least one conducting strand and the at least one hydrophilic strand to provide a patterned flow path.

2. The electrochemical assay device of claim 1, wherein the fabric comprises a sample introduction zone at the proximal end.

3. The electrochemical assay device of claim 1, wherein the at least one conducting strand of the fabric is made of fibers selected from a group comprising cotton, silk, viscose, polypropylene, nylon, or combinations thereof, and wherein the at least one conducting strand is coated with the conducting material in the testing zone.

4. The electrochemical assay device of claim 3, wherein the conducting material is selected from a group comprising carbon ink, gold, platinum, or combinations thereof.

5. The electrochemical assay device of claim 1, wherein the at least one conducting strand is coated with a mediator.

6. The electrochemical assay device of claim 5, wherein the mediator is potassium ferricyanide.

7. The electrochemical assay device of claim 5, wherein the concentration of the conducting material and the mediator is such that the resistance of the at least one conducting strand is less than about 1000 ohms/cm.

8. The electrochemical assay device of claim 1, wherein the plurality of strands further comprise at least one strand coated with a reference conducting material in the testing zone to form a reference electrode.

9. The electrochemical assay device of claim 8, wherein the reference conducting material is a silver/silver halide ink.

10. The electrochemical assay device of claim 1, wherein the plurality of strands further comprise at least one strand coated with the conducting material to form a counter electrode, wherein the concentration of the conducting material is such that the resistance of the counter electrode is less than about 1000 ohms/cm.

11. The electrochemical assay device of claim 1 further comprising a substrate to support the fabric.

12. The electrochemical assay device of claim 11, wherein the substrate is made of a material selected from a group comprising plastic, metal, wood, cardboard, or combinations thereof.

13. The electrochemical assay device of claim 1 further comprising a housing to house the fabric.

14. The electrochemical assay device of claim 13, wherein the housing comprises at least one of a sample introduction port, a transparent viewing port, or combinations thereof.

15. The electrochemical assay device of claim 13, wherein the housing is made of plastic, metal, wood, cardboard, or combinations thereof.

16. The electrochemical assay device of claim 1, wherein the at least one conducting strand is coated with a diagnostic material to provide a working electrode.

17. The electrochemical assay device of claim 16, wherein the diagnostic material is present at a concentration of at least about 1 Unit per active length of the working electrode.

18. The electrochemical assay device of claim 16, wherein the diagnostic material is glucose oxidase.

19. The electrochemical assay device of claim 1, wherein the at least one conducting strand is made of carbon fiber.