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Nucleic acid sequence assembly

  • US 9,715,573 B2
  • Filed: 02/17/2016
  • Issued: 07/25/2017
  • Est. Priority Date: 02/17/2015
  • Status: Active Grant
First Claim
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1. A method for nucleic acid sequence data assembly, comprising:

  • (a) obtaining purified DNA;

    (b) binding the purified DNA with a DNA binding agent to form DNA/chromatin complexes;

    (c) incubating the DNA-chromatin complexes with restriction enzymes to leave sticky ends;

    (d) performing ligation to join ends of DNA;

    (e) sequencing ligated DNA junctions to generate paired end reads;

    (f) obtaining standard paired-end read distance frequency data;

    (g) obtaining grouped contig sequences; and

    (h) scaffolding the grouped contig sequences such that read pair distance frequency data for read pairs that map to separate contigs approximates the standard paired-end read distance frequency data,thereby assembling the sequence data of the nucleic acid.

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