Method of measuring blood component, sensor used in the method, and measuring device

• US 9,719,956 B2
• Filed: 11/12/2015
• Issued: 08/01/2017
• Est. Priority Date: 12/04/2003
• Status: Active Grant

• Current Assignee: PHC Holdings Corporation
• Original Assignee: Panasonic Healthcare Holdings Co., Ltd.
• Inventors: Shinno, Teppei, Ikeda, Shin, Fujiwara, Masaki
• Primary Examiner(s): Kaur, Gurpreet
• Application Number:
  US14/939,281
• Publication Number:
  US 20160077040A1
• Time in Patent Office: 628 Days
• CPC Class Codes: C12Q 1/001 Enzyme electrodes
• CPC Class Codes: C12Q 1/006 for glucose
• CPC Class Codes: G01N 27/3274 Corrective measures, e.g. error detection, compensation for temperature or hematocrit, calibration coding of calibration information G01N33/48771
• CPC Class Codes: G01N 33/66 involving blood sugars, e.g. galactose
• CPC Class Codes: G01N 33/728 Bilirubin; including biliverdin

Abstract

The present invention provides a method of measuring a component in blood, by which an amount of the component can be corrected accurately by measuring a hematocrit (Hct) value of the blood with high accuracy and high reliability and also provides a sensor used in the method. The method of measuring a component in blood using a biosensor comprising a first electrode system including a first working electrode on which at least an oxidoreductase that acts upon the component and a mediator are provided and a first counter electrode and a second working electrode on which the mediator is not provided. The first working electrode and the first counter electrode are used for obtaining the amount of the component and the second working electrode and the first working electrode are used for obtaining the amount of the blood cells.

Claims

1. A method of determining an amount of a component in blood using a biosensor comprising at least two electrodes and a reagent layer comprising a mediator and an oxidoreductase, said reagent layer being disposed on one of said at least two electrodes;

the method comprising;

applying a first voltage between said at least two electrodes such that the electrode on which the reagent layer is disposed acts as a working electrode;

detecting a first current value generated by the application of the first voltage;

applying a second voltage between said at least two electrodes such that the electrode on which the reagent layer is disposed acts as a counter electrode; and

detecting a second current value generated by the application of the second voltage; and

calculating the amount of the component using the first current value and the second current value,

wherein the amount of the component is calculated taking into account an amount of hematocrit present in the blood.

2. The method of claim 1, wherein the first voltage and the second voltage are applied for a time of 0.001 to 10 seconds.