Transmitted light detection type measurement apparatus for skin autofluorescence

• US 9,723,990 B2
• Filed: 06/26/2013
• Issued: 08/08/2017
• Est. Priority Date: 03/21/2012
• Status: Active Grant

• Current Assignee: Korea Electrotechnology Research Institute
• Original Assignee: Korea Electrotechnology Research Institute
• Inventors: Papayan, Garry V, Shin, Il Hyung, Kang, Uk, Lee, Dae Sic, Bae, Soo Jin, Kim, Guang Hoon
• Primary Examiner(s): KISH, JAMES M
• Application Number:
  US13/927,712
• Publication Number:
  US 20140163387A1
• Time in Patent Office: 1,504 Days
• Field of Search: None
• CPC Class Codes: A61B 5/0071 by measuring fluorescence emission
• CPC Class Codes: A61B 5/7225 Details of analog processing, e.g. isolation amplifier, gain or sensitivity adjustment, filtering, baseline or drift compensation A61B5/30, A61B5/316 take precedence; transformers for specific applications H01F38/00; amplifiers H03F

Abstract

The present invention provides a transmitted light detection type measurement apparatus for skin fluorescence, which is configured to perform light irradiation and light detection on a reference sample and a measurement target. The apparatus includes a first light source, a second light source, a first optical detector and a second optical detector, a light source switching controller, and an operator.

Claims

1. A transmitted light type measurement apparatus measuring skin fluorescence, which is configured to perform light irradiation and light detection on a reference sample or a measurement target, the apparatus comprising:

a first light source configured to irradiate excitation light on the measurement target;

a second light source configured to irradiate light, which has a same wavelength range as light generated from the measurement target by the excitation light from the first light source;

a first optical detector disposed to detect transmitted light through the measurement target by the excitation light from the first light source;

a second optical detector disposed to detect the skin fluorescence generated from the measurement target by the excitation light from the first light source and transmitted light passing through the measurement target by light from the second light source;

a light source switching controller configured to control turning on and off of the first light source and the second light source; and

an operation part configured to calculate a skin fluorescence signal by correcting a signal based on the skin fluorescence and the transmitted light detected by the first optical detector and the second optical detector,

wherein the operation part calculates a skin fluorescence value corrected by an equation;

AFcorr=K[I(λ

2,t1)/I₀(λ

2,t1)]/{[T(λ

1)]^k1[T(λ

2)]}^k2

(here,

T(λ

1)=I(λ

1,t1)/I₀(λ

1,t1);

Diffuse transmission coefficient in excitation wavelength;

T(λ

2)=I(λ

2,t2)/I0(λ

2,t2);

Diffuse transmission coefficient in emission wavelength;

I(λ

2,t1);

Inherent fluorescence signal value of skin tissue;

I(λ

1,t1);

Transmitted light signal value of skin tissue in excitation light wavelength;

I(λ

2,t2);

Transmitted light signal value of skin tissue in emission light wavelength;

k1, k2;

Exponents of correction function with respect to excitation light and emission light wavelength;

I0(λ

2,t1);

Inherent fluorescence signal value of reference sample;

I0(λ

1,t1);

Transmitted light signal value of reference sample in excitation light wavelength;

I0(λ

2,t2);

Transmitted light signal value of reference sample in emission light wavelength); and

K;

Ratio coefficient that considers the features of the used reference samples).

2. The transmitted light detection type measurement apparatus of claim 1, further comprising:

a pair of optical transmitters configured to transmit the excitation light irradiated from the first light source and the second light source to the measurement target, transmit the transmitted light to the first optical detector and the second optical detector and transmit the skin fluorescence signal to the second optical detector;

wherein the optical transmitters have a mounting surface mounted with the first light source and the second light source or the first optical detector and the second light source, a reflection surface extending from the mounting surface to the measurement target and reflecting light, and a contact surface connected such that light is incident to the measurement target.

3. The transmitted light detection type measurement apparatus of claim 2, wherein the pair of optical transmitters comprise a first optical prism connected to the first light source and the second light source and a second optical prism connected to the first optical detector and the second optical detector.

4. The transmitted light detection type measurement apparatus of claim 3, wherein the first optical prism and the second optical prism are triangular prisms having a triangular section.

5. The transmitted light detection type measurement apparatus of claim 4, wherein the first optical prism has a mounting surface mounted with the first light source and a reflection surface mounted with the second light source.

6. The transmitted light detection type measurement apparatus of claim 5, wherein the second optical prism has a mounting surface mounted with the first optical detector and a reflection surface mounted with the second optical detector.

7. The transmitted light detection type measurement apparatus of claim 2, wherein the pair of optical transmitters comprise a first optical pipe connected to the first light source and the second light source and a second optical pipe connected to the first optical detector and the second optical detector.

8. The transmitted light detection type measurement apparatus of claim 7, wherein the first optical pipe and the second optical pipe have reflection surfaces that are inclined, and have a tapered pillar shape in which a mounting surface is wider than a contact surface.

9. The transmitted light detection type measurement apparatus of claim 8, wherein the reflection surfaces of the first optical pipe and the second optical pipe are tapered such that a cross-sectional area thereof is reduced from the mounting surface to the contact surface.

10. The transmitted light detection type measurement apparatus of claim 7, wherein the first optical pipe has a mounting surface mounted with the first light source and the second light source.

11. The transmitted light detection type measurement apparatus of claim 10, wherein the mounting surfaces of the first optical pipe and the second optical pipe are inclined to the reflection surface thereof.

12. The transmitted light detection type measurement apparatus of claim 7, wherein the second optical pipe has a mounting surface mounted with the first optical detector and the second optical detector.

13. The transmitted light detection type measurement apparatus of claim 7, further comprising a dichroic prism at a side of the mounting surface of the second optical pipe for dividing detected light into two wavelength bands.

14. The transmitted light detection type measurement apparatus of claim 13, where the first optical detector is disposed to detect reflected light from the dichroic prism, and the second optical detector is disposed to detect transmitted light from the dichroic prism.

15. The transmitted light detection type measurement apparatus of claim 7, wherein the first optical pipe and the second optical pipe are vertical-type optical pipes that extend in a perpendicular direction to the contact surface with the measurement target.

16. The transmitted light detection type measurement apparatus of claim 7, wherein the first optical pipe and the second optical pipe are horizontal-type optical pipes that extend in a parallel direction to the contact surface with the measurement target.

17. The transmitted light detection type measurement apparatus of claim 16, wherein the reflection surfaces of the first optical pipe and the second optical pipe comprise a bent reflection surface that is inclined to the contact surface, respectively.

18. The transmitted light detection type measurement apparatus of claim 16, wherein the reflection surfaces of the first optical pipe and the second optical pipe are processed by mirror coating.

19. The transmitted light detection type measurement apparatus of claim 2, further comprising:

a transfer part configured to move the optical transmitter in a vertical direction; and

a thickness indicator configured to measure a distance between the two optical transmitters and indicating a thickness of the measurement target.

20. The transmitted light detection type measurement apparatus of claim 2, further comprising an optical connector disposed over the contact surface of the optical transmitter and contacting the measurement target.

21. The transmitted light detection type measurement apparatus of claim 20, wherein the optical connector serves as a connection layer formed of a liquid material or an elastic material between the optical transmitter and the measurement target.

22. The transmitted light detection type measurement apparatus of claim 1, wherein the light source switching controller is further configured to control the first light source and the second light source such that turning-on states of the first light source and the second light source are separated from each other in time.

23. The transmitted light detection type measurement apparatus of claim 22, wherein the switching controller is further configured to detect the skin fluorescence signal and the transmitted light signal from the first light source and the transmitted light signal from the second light source while continuously repeating a process of sequentially turning on and off the first light source and the second light source.

24. The transmitted light detection type measurement apparatus of claim 1, wherein the measurement target and the reference sample are selectively located on optical paths of the first light source and the second light source.

25. The transmitted light detection type measurement apparatus of claim 1, wherein the first light source irradiates light with a wavelength of 370±

20 nm.

26. The transmitted light detection type measurement apparatus of claim 1, wherein the second light source irradiates light with a wavelength of 440±

20 nm.

27. The transmitted light detection type measurement apparatus of claim 1, wherein the switching controller is further configured to control all the first light source and the second light source to be turned off before turning on each of the light sources.

28. The transmitted light detection type measurement apparatus of claim 27, wherein when the switching controller turns off all the first light source and the second light source, the first optical detector and the second optical detector measure dark signals, and the operation part stores the measured dark signals and compensate for the skin fluorescence signal and the transmitted light signal detected from the stored dark signals.

29. The transmitted light detection type measurement apparatus of claim 1, wherein the switching controller is further configured to control the first light source and the second light source to repeat turning on and off at a period of about 10 Hz to about 100 Hz.

30. The transmitted light detection type measurement apparatus of claim 1, further comprising an optical detector switching controller for controlling turning on and off of the first optical detector and the second optical detector.

31. The transmitted light detection type measurement apparatus of claim 1, comprising:

an optical sensor comprising the first light source, the second light source, the first optical detector, and the second optical detector; and

a main body electrically connected to the optical sensor and comprising the operation part, wherein the optical sensor is detachable from the main body.

32. The transmitted light detection type measurement apparatus of claim 31, wherein the optical sensor comprises a memory for storing detected data.

33. The transmitted light detection type measurement apparatus of claim 1, comprising:

an optical sensor comprising the first light source, the second light source, the first optical detector, and the second optical detector; and

a main body electrically connected to the optical sensor and comprising the operation part, wherein the optical sensor is detachable from the main body;

wherein the optical sensor comprises a first fixing part connected to the first light source and the second light source and a second fixing part connected to the first optical detector and the second optical detector, and the first and second fixing parts face each other to form an insertion space therebetween.

34. The transmitted light detection type measurement apparatus of claim 33, wherein the first light source and the second light source are disposed at a distal end of the first fixing part to directly irradiate light on the measurement target, and the first optical detector and the second detector are disposed at a distal end of the second fixing part to directly detect the transmitted light and the skin fluorescence.

35. The transmitted light detection type measurement apparatus of claim 34, wherein the first optical detector and the second optical detector are configured to form two sectors, and comprise band-pass filters at the front of the two sectors to divide light into a first wavelength (λ

1) and a second wavelength (λ

2), respectively.

36. The transmitted light detection type measurement apparatus of claim 34, wherein the first fixing part and the second fixing part are manufactured in a form of a clip to fix the measurement target while compressing the measurement target.

37. The transmitted light detection type measurement apparatus of claim 33, wherein the reference sample is movably mounted in the optical sensor, and is loaded into the insertion space after the measurement target is removed from the insertion space between the first fixing part and the second fixing part.

38. The transmitted light detection type measurement apparatus of claim 37, wherein the optical sensor performs measurement on a skin when the skin that is the measurement target is located at the insertion space, and performs measurement on the reference sample when the measurement target is removed and then the reference sample is located at the insertion space.

39. The transmitted light detection type measurement apparatus of claim 38, wherein the optical sensor stores measured results about the skin that is the measurement target and the reference sample, and transmits stored data about the skin and reference sample to the main body to allow the operation part to calculate the corrected skin fluorescence signals.

40. The transmitted light detection type measurement apparatus of claim 1, comprising:

an optical sensor comprising the first light source, the second light source, the first optical detector, and the second optical detector; and

a main body electrically connected to the optical sensor and comprising the operation part, wherein the optical sensor is detachable from the main body;

wherein the optical sensor comprises a common light source light guide for transmitting light irradiated from the first light source and the second light source in a shared manner.

41. The transmitted light detection type measurement apparatus of claim 40, wherein the optical sensor comprises a common detection light guide for transmitting the transmitted light and the skin fluorescence to the first optical detector and the second optical detector in a shared manner.

42. The transmitted light detection type measurement apparatus of claim 40, wherein the optical sensor comprises a first dichroic mirror on an optical path to transmit light irradiated from the first light source and the second light source to the common light source light guide.

43. The transmitted light detection type measurement apparatus of claim 42, wherein the optical sensor comprises a second dichroic mirror on an optical path to divide light from the common detection light guide and transmit the divided light to the first optical detector and the second optical detector.

44. The transmitted light detection type measurement apparatus of claim 43, further comprising a first detection filter between the second dichroic mirror and the first optical detector and a second detection filter between the second dichroic mirror and the second optical detector, wherein the first detection filter passes light of a first wavelength and inhibits light of a second wavelength, and the second detection filter inhibits light of the first wavelength and passes light of the second wavelength.

45. The transmitted light detection type measurement apparatus of claim 43, further comprising objective lenses between the first and second optical detectors and the second dichroic mirror to concentrate light passing the second dichroic mirror on the first optical detector and the second optical detector, respectively.

46. The transmitted light detection type measurement apparatus of claim 42, further comprising a light source filter between the first light source and the first dichroic mirror to pass light of a first wavelength irradiated from the first light source and inhibit light of a second wavelength irradiated from the second light source.

47. The transmitted light detection type measurement apparatus of claim 42, further comprising objective lenses between the first and second light sources and the first dichroic mirror to condense light irradiated from the first and second light sources, respectively.

48. The transmitted light detection type measurement apparatus of claim 1, comprising:

an optical sensor comprising the first light source, the second light source, the first optical detector, and the second optical detector;

a main body electrically connected to the optical sensor and comprising the operation part, wherein the optical sensor is detachable from the main body; and

a first light source light guide for transmitting light irradiated from the first light source and a second light source light guide for transmitting light irradiated from the second light source.

49. The transmitted light detection type measurement apparatus of claim 48, wherein the optical sensor comprises a first detection light guide for transmitting the transmitted light of the first light source to the first optical detector and a second detection light guide for transmitting the transmitted light of the second light source or the skin fluorescence to the second optical detector.

50. The transmitted light detection type measurement apparatus of claim 49, further comprising a first detection filter between the first light source light guide and the first optical detector to pass light of a first wavelength and inhibit light of a second wavelength, and a second detection filter between the second light source light guide and the second optical detector to inhibit light of the first wavelength and pass light of the second wavelength.

51. The transmitted light detection type measurement apparatus of claim 48, further comprising an light source filter between the first light source and the first light source light guide to pass light of a first wavelength irradiated from the first light source and inhibit light of a second wavelength irradiated from the second light source.

52. The transmitted light detection type measurement apparatus of claim 1, wherein the main body further comprises a display part, and the display part outputs the corrected skin fluorescence signals calculated in the operation part.