Microelectrodes in an ophthalmic electrochemical sensor

• US 9,724,027 B2
• Filed: 05/15/2015
• Issued: 08/08/2017
• Est. Priority Date: 10/12/2012
• Status: Active Grant

• Current Assignee: Verily Life Sciences LLC (Alphabet Inc.)
• Original Assignee: Verily Life Sciences LLC (Alphabet Inc.)
• Inventors: Liu, Zenghe
• Primary Examiner(s): Natnithithadha, Navin
• Assistant Examiner(s): MESSERSMITH, ERIC J
• Application Number:
  US14/713,968
• Publication Number:
  US 20150245792A1
• Time in Patent Office: 816 Days
• CPC Class Codes: A61B 5/0002 Remote monitoring of patients using telemetry, e.g. transmission of vital signals via a communication network A61B5/07 takes precedence; transmission systems for measured values G08C; transmission H04B; transmission of digital information H04L; wireless communication networks H04W
• CPC Class Codes: A61B 5/0004 characterised by the type of physiological signal transmitted
• CPC Class Codes: A61B 5/14507 specially adapted for measuring characteristics of body fluids other than blood devices for taking samples of body liquids A61B10/0045
• CPC Class Codes: A61B 5/14532 for measuring glucose, e.g. by tissue impedance measurement
• CPC Class Codes: A61B 5/14546 for measuring analytes not otherwise provided for, e.g. ions, cytochromes
• CPC Class Codes: A61B 5/1468 using chemical or electrochemical methods, e.g. by polarographic means A61B5/1486 takes precedence
• CPC Class Codes: A61B 5/1473 invasive, e.g. introduced into the body by a catheter A61B5/1482 takes precedence
• CPC Class Codes: A61B 5/1486 using enzyme electrodes, e.g. with immobilised oxidase
• CPC Class Codes: A61B 5/6821 Eye
• CPC Class Codes: A61B 5/6867 specially adapted to be attached or implanted in a specific body part A61B5/6847 takes precedence
• CPC Class Codes: G01N 27/3271 Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood amperometry per se G01N27/49; aspects concerning the enzyme reagent C12Q1/001
• CPC Class Codes: G02C 7/04 Contact lenses for the eyes disinfection or sterilisation of contact lenses A61L12/00

Abstract

An eye-mountable device includes an electrochemical sensor embedded in a polymeric material configured for mounting in front of a surface of an eye. The electrochemical sensor includes a working electrode, a reference electrode, and a reagent that selectively reacts with an analyte to generate a sensor measurement related to a concentration of the analyte in a fluid to which the eye-mountable device is exposed. The working electrode can have at least one dimension less than 25 micrometers. The reference electrode can have an area at least five times greater than an area of the working electrode. A portion of the polymeric material can surround the working electrode and the reference electrode such that an electrical current conveyed between the working electrode and the reference electrode is passed through the at least partially surrounding portion of the transparent polymeric material.

Claims

1. An eye-mountable device, comprising:

a transparent polymeric material having an eye-facing surface and an outward facing surface, wherein the transparent polymeric material is configured to be mounted in front of an eye surface, and wherein the transparent polymeric material includes a channel in the outward facing surface;

a substrate at least partially embedded within the polymeric material;

an antenna disposed on the substrate;

a two-electrode electrochemical sensor disposed on a surface of the substrate and including;

a working electrode having a width dimension equal to or less than 25 micrometers, wherein the width dimension is substantially parallel to the surface of the substrate; and

a reference electrode having an area at least five times greater than an area of the working electrode; and

a controller electrically connected to the electrochemical sensor and the antenna, wherein the controller is configured to;

(i) apply a voltage between the working electrode and the reference electrode sufficient to generate an amperometric current related to the concentration of an analyte in a fluid to which the eye-mountable device is exposed;

(ii) measure the amperometric current; and

(iii) use the antenna to indicate the measured amperometric current,

wherein the working electrode and the reference electrode are both exposed by the channel to provide direct contact with the fluid.

2. The eye-mountable device according to claim 1, wherein the channel is configured to fluidly connect an outer tear film layer to the working electrode and reference electrode while the transparent polymeric material is mounted in front of the eye surface.

3. The eye-mountable device according to claim 1, wherein the width dimension of the working electrode is approximately equal to 10 micrometers.

4. The eye-mountable device according to claim 1, wherein the width dimension of the working electrode is less than 10 micrometers.

5. The eye-mountable device according to claim 1, wherein the working electrode and the reference electrode are each disposed on the substrate so as to be approximately coplanar.

6. The eye-mountable device according to claim 1, further comprising a reagent that selectively reacts with the analyte, wherein the reagent is localized proximate the working electrode.

7. The eye-mountable device according to claim 6, wherein the reagent is localized away from the reference electrode.

8. The eye-mountable device according to claim 1, further comprising a power supply disposed on the substrate and electrically connected to the antenna and the controller, wherein the power supply is configured to convert power from radio frequency radiation received by the antenna into electrical power and to supply the electrical power to the controller.

9. The eye-mountable device according to claim 1, wherein the controller is configured to indicate the measured amperometric current by modulating an impedance of the antenna.

10. The eye-mountable device according to claim 1, wherein the electrochemical sensor is situated on a mounting surface of the substrate proximate the outward facing surface of the polymeric material.

11. The eye-mountable device according to claim 1, wherein the polymeric material is a substantially transparent vision correction lens and is shaped to provide a predetermined vision-correcting optical power.

12. A system comprising:

an eye-mountable device including;

a transparent polymeric material having an eye-facing surface and an outward facing surface, wherein the transparent polymeric material is configured to be mounted in front of an eye surface, and wherein the transparent polymeric material includes a channel in the outward facing surface;

a substrate at least partially embedded within the polymeric material;

an antenna disposed on the substrate;

a two-electrode electrochemical sensor disposed on a surface of the substrate and including;

a working electrode having a width dimension equal to or less than 25 micrometers, wherein the width dimension is substantially parallel to the surface of the substrate; and

a reference electrode having an area at least five times greater than an area of the working electrode; and

a controller electrically connected to the electrochemical sensor and the antenna, wherein the controller is configured to;

(i) apply a voltage between the working electrode and the reference electrode sufficient to generate an amperometric current related to the concentration of an analyte in a fluid to which the eye-mountable device is exposed;

(ii) measure the amperometric current; and

(iii) use the antenna to indicate the measured amperometric current,

wherein the working electrode and the reference electrode are both exposed by the channel to provide direct contact with the fluid such that operation of the two-electrode electrochemical sensor is substantially unaffected by a permeability of the transparent polymeric material to the analyte; and

a reader including;

one or more antennae configured to;

transmit radio frequency radiation to power the eye-mountable device, and

receive indications of the measured amperometric current via backscatter radiation received at the one or more antennae; and

a processing system configured to determine a tear film analyte concentration value based on the backscatter radiation.

13. The system according to claim 12, wherein the channel is configured to fluidly connect an outer tear film layer to the working electrode and reference electrode while the transparent polymeric material is mounted in front of the eye surface.

14. The system according to claim 12, wherein the width dimension of the working electrode is approximately equal to 10 micrometers.

15. The system according to claim 12, wherein the width dimension of the working electrode is a microelectrode having at least one dimension less than 10 micrometers.

16. The system according to claim 12, wherein the working electrode and the reference electrode are each disposed on the substrate so as to be approximately coplanar.

17. The system according to claim 12, further comprising a reagent that selectively reacts with the analyte, wherein the reagent is localized proximate the working electrode.

18. The system according to claim 12, wherein the eye-mountable device further includes a power supply disposed on the substrate and electrically connected to the antenna and the controller, wherein the power supply is configured to convert power from radio frequency radiation received by the antenna into electrical power and to supply the electrical power to the controller,

wherein the controller is configured to indicate the measured amperometric current by adjusting an impedance of the antenna included in the eye-mountable device, and

wherein the reader is configured to wirelessly sense the impedance of the antenna.

19. A method comprising:

applying a voltage between a working electrode and a reference electrode sufficient to cause electrochemical reactions at the working electrode, wherein the working electrode and the reference electrode are disposed on a surface of a substrate embedded within an eye-mountable device that includes a transparent polymeric material having an eye-facing surface and an outward facing surface, wherein the transparent polymeric material is configured to be mounted in front of an eye surface, and wherein the transparent polymeric material includes a channel in the outward facing surface, wherein the working electrode has a width dimension equal to or less than 25 micrometers, wherein the width dimension is substantially parallel to the surface of the substrate, wherein the reference electrode has an area at least five times greater than an area of the working electrode, and wherein the working electrode and the reference electrode are both exposed by the channel to a fluid such that the electrochemical reactions are related to a concentration of an analyte in the fluid, wherein the fluid enters the channel and forms direct contact with both of the working and reference electrodes;

while applying the voltage, measuring an amperometric current through the working electrode; and

wirelessly indicating the measured amperometric current via an antenna embedded within the eye-mountable device.

20. The method according to claim 19, wherein the width dimension of the working electrode is less than or approximately equal to 10 micrometers.