×

Therapeutic gene-switch constructs and bioreactors for the expression of biotherapeutic molecules, and uses thereof

  • US 9,724,430 B2
  • Filed: 09/24/2015
  • Issued: 08/08/2017
  • Est. Priority Date: 09/28/2007
  • Status: Active Grant
First Claim
Patent Images

1. A composition comprising:

  • (1) a first polynucleotide encoding a gene switch, said gene switch comprising at least one transcription factor sequence, wherein said at least one transcription factor sequence encodes a ligand-dependent transcription factor in operable association with a hypoxia-inducible promoter, wherein the hypoxia-inducible promoter is a nucleic acid sequence at least 95% identical to the sequence from nucleotide 4958 to nucleotide 5535 of SEQ ID NO;

    7, and(2) a second polynucleotide encoding a therapeutic polypeptide or therapeutic polynucleotide operably associated with a transcription factor-regulated promoter which is activated by said ligand-dependent transcription factor, wherein a ligand is capable of inducing expression of said therapeutic polypeptide or therapeutic polynucleotide, wherein said composition is for use in treating, ameliorating, or preventing a disease or disorder.

View all claims
  • 0 Assignments
Timeline View
Assignment View
    ×
    ×