Formulations and methods for stabilizing PCR reagents
First Claim
1. A liquid composition comprising a polymerase and a storage stabilizing agent, wherein the storage stabilizing agent comprises at least one cationic ester compound selected from the group consisting of:
- [i] 1-(2-ethoxy-2-oxoethyl)-3-hydroxy-1-methylpiperidium bromide,[ii] N-(2-cyanoethyl)-2-ethoxy-N,N-dimethyl-2-oxoethanaminium bromide,[iii] 2-ethoxy-N,N-diethyl-N-methyl-2-oxoethanaminium bromide,[iv] N-(2-ethoxy-2-oxoethyl)-N,N-dimethylcyclohexanaminium bromide, and[v] 2-ethoxy-N-(2-(2-hydroxyethoxy)ethyl)-N,N-dimethyl-2-oxoethanaminium bromide.
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Abstract
Described herein are stabilized polymerase compositions comprising a polymerase and an polymerase stabilizing agent, such as a non-detergent zwitterionic stabilizer or a cationic ester disclosed, for use in nucleic acid amplification or nucleic acid sequencing. Compositions are provided for the stabilization of one or more polymerases in a single stabilized liquid formulation. Also disclosed are methods for making and using stabilized polymerase compositions and kits for nucleic acid amplification and sequencing comprising the stabilized polymerase compositions provided.
316 Citations
11 Claims
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1. A liquid composition comprising a polymerase and a storage stabilizing agent, wherein the storage stabilizing agent comprises at least one cationic ester compound selected from the group consisting of:
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[i] 1-(2-ethoxy-2-oxoethyl)-3-hydroxy-1-methylpiperidium bromide, [ii] N-(2-cyanoethyl)-2-ethoxy-N,N-dimethyl-2-oxoethanaminium bromide, [iii] 2-ethoxy-N,N-diethyl-N-methyl-2-oxoethanaminium bromide, [iv] N-(2-ethoxy-2-oxoethyl)-N,N-dimethylcyclohexanaminium bromide, and [v] 2-ethoxy-N-(2-(2-hydroxyethoxy)ethyl)-N,N-dimethyl-2-oxoethanaminium bromide. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
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Specification