High efficiency ethanol process and high protein feed co-product

• US 9,725,742 B2
• Filed: 05/09/2013
• Issued: 08/08/2017
• Est. Priority Date: 05/10/2012
• Status: Expired due to Fees

• Current Assignee: Abengoa Bioenergy New Technologies, LLC (Abengoa SA)
• Original Assignee: Abengoa Bioenergy New Technologies, LLC (Abengoa SA)
• Inventors: Escudero, Carlos Blzquez, Gmez, Pablo Gutirrez
• Primary Examiner(s): Singh, Satyendra K
• Application Number:
  US14/399,630
• Publication Number:
  US 20150118727A1
• Time in Patent Office: 1,552 Days
• CPC Class Codes: A23K 10/38 from distillers' or brewers' waste
• CPC Class Codes: C07C 29/80 by distillation
• CPC Class Codes: C12P 19/14 produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase , e.g. by cellulase, hemicellulase
• CPC Class Codes: C12P 2201/00 Pretreatment of cellulosic or lignocellulosic material for subsequent enzymatic treatment or hydrolysis
• CPC Class Codes: C12P 2203/00 Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source
• CPC Class Codes: C12P 7/06 Ethanol, i.e. non-beverage
• CPC Class Codes: C12P 7/10 substrate containing cellulosic material
• CPC Class Codes: C12P 7/14 Multiple stages of fermentation; Multiple types of microorganisms or re-use of microorganisms
• CPC Class Codes: Y02E 50/10 Biofuels, e.g. bio-diesel
• CPC Class Codes: Y02P 60/87 Re-use of by-products of food processing for fodder production

Abstract

A process for obtaining high ethanol yield from the fermentation of an energy crop and for producing a nutritionally enhanced feed co-product is provided. In particular, the process includes converting non-fermentable polysaccharides in an energy crop into fermentable sugar. The fermentable sugars may be fermented into ethanol thereby enhancing the ethanol yield. In addition, separation of ethanol from the fermentation product yields a whole stillage product having enhanced protein content and reduced fiber content. The process requires little or no modification to the configuration of existing commercial ethanol facilities.

Claims

1. A process for producing ethanol, the process comprising:

forming an acidic aqueous medium comprising a plant matter feedstock and having a pH of from about 2 to about 6, wherein the plant matter comprises starch and another polysaccharide selected from the group consisting of cellulose, hemicellulose, and combinations thereof;

hydrolyzing at least a portion of the starch, the another polysaccharide, or both in the acidic aqueous medium at a temperature of at least 85°

C.;

contacting at least a portion of the starch in the acidic aqueous medium with an α

-amylase, which catalyzes enzymatic hydrolysis of at least a portion of the starch to yield an enzymatic hydrolysate containing simple sugars having from one to three saccharide units; and

contacting the enzymatic hydrolysate in a simultaneous saccharification-fermentation (SSF) zone with a yeast, a glucoamylase, and a polysaccharide enzyme selected from the group consisting of cellulase, hemicellulase, and combinations thereof to form a saccharification-fermentation mixture, wherein the polysaccharide enzyme catalyzes the hydrolysis of at least a portion of the another polysaccharide to simple sugars having from one to four saccharide units, wherein said polysaccharide enzyme is introduced into the SSF zone after introduction of the yeast and glucoamylase into the SSF zone, wherein during a saccharification-fermentation period at least a portion of the simple sugars derived from the starch are converted by fermentation to produce ethanol within the SSF zone and at least a portion of the simple sugars derived from the another polysaccharide are converted by fermentation to produce ethanol within the SSF zone, wherein said polysaccharide enzyme is introduced at a point within from about 10% to about 80% of the duration of the saccharification-fermentation period; and

wherein the process provides an ethanol yield increase of at least 2% as compared to the yield for a same process for producing ethanol that does not include introduction of said polysaccharide enzyme into the SSF zone.

2. The process of claim 1 further comprising:

contacting at least a portion of the aqueous medium in a liquefaction zone with a polysaccharide enzyme selected from the group consisting of cellulase, hemicellulase, and combinations thereof, wherein the polysaccharide enzyme catalyzes the hydrolysis of at least a portion of the another polysaccharide to simple sugars having from one to four saccharide units.

3. The process of claim 1 further comprising:

contacting the enzymatic hydrolysate with a polysaccharide enzyme selected from the group consisting of cellulase, hemicellulase, and combinations thereof and reducing the temperature of the enzymatic hydrolysate in a cooling zone, wherein the polysaccharide enzyme catalyzes the hydrolysis of at least a portion of the another polysaccharide to simple sugars having from one to four saccharide units.

4. The process of claim 1 wherein the plant matter feedstock comprises milled fruits or seeds of an energy crop.

5. The process of claim 4 wherein the plant matter feedstock comprises corn, wheat, barley or mixtures thereof.

6. The process of claim 5 wherein the plant matter feedstock comprises barley.

7. The process of claim 1 wherein the temperature of the enzymatic hydrolysate is reduced prior to contacting with the polysaccharide enzyme.

8. The process of claim 1 wherein the temperature of the saccharification-fermentation mixture in the SSF zone is from about 25°

C. to about 35°

C.

9. The process of claim 1 wherein the polysaccharide enzyme comprises a cellulase.

10. The process of claim 1 wherein the polysaccharide enzyme comprises a cellulase and a hemicellulase.

11. The process of claim 1 wherein the yeast comprises a C₆ sugar yeast and a C₅ sugar yeast.

12. The process of claim 1 wherein the yeast is capable of converting C₆ sugars and C₅ sugars to ethanol.

13. The process of claim 1 wherein the process further comprises:

distilling the saccharification-fermentation mixture to separate at least a portion of the ethanol thereby forming;

(i) a distillate product comprising ethanol and (ii) a bottoms product comprising whole stillage, and obtaining a modified co-product from the whole stillage wherein the modified co-product has a fiber content of from about 25 wt. % to about 45 wt. % and a protein content of from about 26 wt. % to about 40 wt. %.

14. The process of claim 13 wherein an ethanol yield of from about 200 to about 450 liters ethanol per metric ton of plant matter feedstock is achieved.

15. The process of claim 13 wherein the plant matter feedstock comprises

(1) barley and the modified feed co-product comprises:

Protein;

28-35 wt. % dry matter basis

Fats;

2-10 wt. % dry matter basis

Fiber;

35-45 wt. % dry matter basis

Ash;

2-10 wt. % dry matter basis;

(2) wheat and the modified feed co-product comprises;

Protein;

30-35 wt. % dry matter basis

Fats;

2-10 wt. % dry matter basis

Fiber;

35-40 wt. % dry matter basis

Ash;

2-10 wt. % dry matter basis;

or

(3) corn and the modified feed co-product comprises;

Protein;

30-35 wt. % dry matter basis

Fats;

5-20 wt. % dry matter basis

Fiber;

30-36 wt. % dry matter basis

Ash;

2-10 wt. % dry matter basis.

16. The process of claim 13 wherein the process further comprises:

forming an acidic aqueous medium comprising the whole stillage or derivative thereof and having a pH from about 2 to about 6, wherein the whole stillage or derivative thereof comprises starch and another polysaccharide selected from the group consisting of hemicellulose and cellulose;

hydrolyzing at least a portion of the starch, the another polysaccharide, or both in the acidic aqueous medium a temperature of at least 85°

C.;

contacting at least a portion of the starch, the another polysaccharide, or both in the acidic aqueous medium with an enzyme selected from the group consisting of a-amylase, cellulase, hemicellulase, and combinations thereof, the enzyme catalyzing enzymatic hydrolysis of at least a portion of the starch, the another polysaccharide, or both into a fermentable sugar;

contacting the fermentable sugars with a yeast, wherein during a fermentation period at least a portion of the simple sugars derived from the starch and/or the another polysaccharide are converted by fermentation to produce ethanol; and

distilling the fermentation mixture to separate at least a portion of the ethanol thereby forming;

(i) a secondary distillate product comprising ethanol; and

(ii) a secondary bottoms product comprising a secondary whole stillage.

17. The process of claim 16 wherein a secondary modified feed co-product is obtained from the secondary whole stillage.

18. The process of claim 1 wherein the plant matter feedstock comprises a product derived from fermentation of fruit or seeds of an energy crop to produce ethanol, the product selected from the group consisting of whole stillage, thin stillage, condensates of whole stillage, and condensates of thin stillage.

19. The process of claim 1 wherein the acidic aqueous medium comprising the plant matter feed stock is hydrolyzed at a temperature of between about 120°

C. and about 160°

C.

20. The process of claim 1 wherein the process provides an ethanol yield increase of from 2% to 15% as compared to the yield for a same process for producing ethanol that does not include a polysaccharide enzyme selected from the group consisting of cellulase, hemicellulase, and combinations thereof.