Linear valve arrays

• US 9,731,266 B2
• Filed: 07/21/2015
• Issued: 08/15/2017
• Est. Priority Date: 08/20/2010
• Status: Active Grant

• Current Assignee: IntegenX Inc. (Thermo Fisher Scientific Incorporated)
• Original Assignee: IntegenX Inc. (Thermo Fisher Scientific Incorporated)
• Inventors: Jovanovich, Stevan B., Stern, Seth
• Primary Examiner(s): FORMAN, BETTY J
• Application Number:
  US14/804,675
• Publication Number:
  US 20160016140A1
• Time in Patent Office: 756 Days
• Field of Search: None
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00389 Feeding through valves
• CPC Class Codes: B01J 2219/00599 Solution-phase processes
• CPC Class Codes: B01J 2219/00716 Heat activated synthesis
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01L 2200/0647 Handling flowable solids, e.g. microscopic beads, cells, particles
• CPC Class Codes: B01L 2200/10 Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
• CPC Class Codes: B01L 2200/16 Reagents, handling or storing thereof
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0867 Multiple inlets and one sample wells, e.g. mixing, dilution
• CPC Class Codes: B01L 2300/087 Multiple sequential chambers
• CPC Class Codes: B01L 2400/043 magnetic forces
• CPC Class Codes: B01L 2400/0481 squeezing of channels or chambers
• CPC Class Codes: B01L 2400/0655 pinch valves
• CPC Class Codes: B01L 3/502738 characterised by integrated valves microvalves F16K99/0001
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046
• CPC Class Codes: C12Q 1/6806 Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay C12Q1/6804 takes precedence
• CPC Class Codes: C12Q 1/6869 Methods for sequencing
• CPC Class Codes: C12Q 1/6874 involving nucleic acid arrays, e.g. sequencing by hybridisation
• CPC Class Codes: F16K 2099/0084 Chemistry or biology, e.g. "lab-on-a-chip" technology
• CPC Class Codes: F16K 99/0015 : Diaphragm or membrane valves
• CPC Class Codes: F16K 99/0059 : actuated by a pilot fluid

Abstract

The invention provides systems, devices, methods, and kits for performing an integrated analysis. The integrated analysis can include sample processing, library construction, amplification, and sequencing. The integrated analysis can be performed within one or more modules that are fluidically connected to each other. The one or more modules can be controlled and/or automated by a computer. The integrated analysis can be performed on a tissue sample, a clinical sample, or an environmental sample. The integrated analysis system can have a compact format and return results within a designated period of time.

Claims

1. A device comprising:

one or more modules, wherein each of said one or more modules comprises;

(a) one or more valve arrays, each comprising a series of valves connected through fluidic connections, each of said series of valves comprising, in order, a first terminal valve, at least three intermediate valves and a second terminal valve;

(b) a plurality of first ports, each fluidically connected through a separate control valve to a common rail, said common rail fluidically connected to each of the first terminal valves of said valve arrays; and

(c) a plurality of second ports, each fluidically connected through a separate control valve to a common rail, said common rail fluidically connected to each of the second terminal valves of said valve arrays,

wherein each of said first terminal valves, said second terminal valves and said intermediate valves of said valve arrays comprises a valve with a valve chamber.

2. The device of claim 1 wherein the fluidic connections are unbranched.

3. The device of claim 1, wherein each of said valve arrays further comprises a movable magnet that is adjacent to a given valve in said series of valves.

4. The device of claim 1, wherein each of said modules further comprises at least one port that is fluidically connected through a separate control valve to each of said first terminal valves.

5. The device of claim 4, wherein said at least one port is fluidically connected to a temperature-controlled channel.

6. The device of claim 4, wherein each of said modules further comprises at least one additional port that is fluidically connected through a separate control valve to each of said second terminal valves.

7. The device of claim 1, wherein said one or more valve arrays is a plurality of valve arrays.

8. The device of claim 1, wherein said one or more modules is a plurality of modules in fluidic connection with each other.

9. The device of claim 8, further comprising at least one additional common rail, wherein said plurality of modules is in fluidic connection with each other through said at least one additional common rail.

10. The device of claim 9, wherein said at least one additional common rail comprises a plurality of additional common rails, and wherein said plurality of modules is in fluidic connection with each other through said plurality of additional common rails.

11. The device of claim 10, wherein each of said first terminal valves is in fluidic connection with a first subset of said plurality of additional common rails.

12. The device of claim 10, wherein each of said second terminal valves is in fluidic connection with a second subset of said plurality of additional common rails.

13. The device of claim 8, wherein each of said plurality of modules comprises a plurality of valve arrays.

14. The device of claim 1, further comprising an actuating module configured to actuate said valve arrays.