Fluorophore chelated lanthanide luminescent probes with improved quantum efficiency
First Claim
Patent Images
1. An in vitro method of detecting a target with a probe, the method comprising:
- contacting a sample with a luminescent probe composition comprising a targeting-probe moiety having an affinity for a target, the targeting-probe moiety covalently conjugated to a luminescent chelate composition comprising;
(i) a fluorophore of Formula (I) or Formula (II); and
(ii) a chelating moiety covalently joined, optionally through a first linker, to the fluorophore,wherein Formula (I) is;
2 Assignments
0 Petitions
Accused Products
Abstract
The invention relates to novel luminescent compositions of matter containing a fluorophore, synthetic methods for making the compositions, macromolecular conjugates of the compositions, and the use of the compositions in various methods of detection. The invention also provides kits containing the compositions and their conjugates for use in the methods of detection.
-
Citations
9 Claims
-
1. An in vitro method of detecting a target with a probe, the method comprising:
-
contacting a sample with a luminescent probe composition comprising a targeting-probe moiety having an affinity for a target, the targeting-probe moiety covalently conjugated to a luminescent chelate composition comprising; (i) a fluorophore of Formula (I) or Formula (II); and (ii) a chelating moiety covalently joined, optionally through a first linker, to the fluorophore, wherein Formula (I) is; - View Dependent Claims (3)
-
-
2. An in vivo method of detecting a target with a probe, the method comprising:
-
administering to a subject a luminescent probe composition comprising a target-probe moiety having an affinity for a target, the targeting-probe moiety covalently conjugated to a luminescent chelate composition comprising; (i) a fluorophore of Formula (I) or Formula (II); and (ii) a chelating moiety covalently joined, optionally through a first linker, to the fluorophore, wherein Formula (I) is; - View Dependent Claims (4)
-
-
5. A method for detecting an analyte in a sample comprising the steps of:
-
(a) contacting a sample containing the analyte with a luminescent chelate composition to specifically bind the analyte to form a luminescent-analyte complex; (b) illuminating the sample with excitation radiation; and (c) detecting emission radiation of at least one luminescent-analyte complex; wherein the luminescent chelate composition comprises; (i) a fluorophore of Formula (I) or Formula (II); and (ii) a chelating moiety covalently joined, optionally through a first linker, to the fluorophore; wherein Formula (I) is; - View Dependent Claims (6, 7, 8, 9)
-
Specification