Light-absorbing compositions and methods of use

• US 9,737,472 B2
• Filed: 12/07/2015
• Issued: 08/22/2017
• Est. Priority Date: 08/09/2010
• Status: Active Grant

• Current Assignee: Allele Biotechnology & Pharmaceuticals, Inc.
• Original Assignee: Allele Biotechnology & Pharmaceuticals, Inc.
• Inventors: Wang, Jiwu
• Primary Examiner(s): Gudibande, Satyanarayana R
• Assistant Examiner(s): Lee, Jia-Hai
• Application Number:
  US14/961,678
• Publication Number:
  US 20160193132A1
• Time in Patent Office: 624 Days
• Field of Search: None
• CPC Class Codes: A61K 2800/434 Luminescent, Fluorescent; Optical brighteners; Photosensitizers
• CPC Class Codes: A61K 49/0021 the fluorescent group being a small organic molecule
• CPC Class Codes: A61K 49/0047 Green fluorescent protein [GFP]
• CPC Class Codes: A61K 8/64 Proteins; Peptides; Derivatives or degradation products thereof
• CPC Class Codes: A61K 8/987 of species other than mammals or birds
• CPC Class Codes: A61Q 17/04 Topical preparations for affording protection against sunlight or other radiation; Topical sun tanning preparations
• CPC Class Codes: C07K 14/43504 from invertebrates
• CPC Class Codes: C09C 2210/50 Fluorescent, luminescent or photoluminescent properties
• CPC Class Codes: C09J 2489/00 Presence of protein
• CPC Class Codes: C12N 15/8212 Colour markers, e.g. beta-glucoronidase [GUS], green fluorescent protein [GFP], carotenoid
• CPC Class Codes: C12N 2310/3513 Protein; Peptide

Abstract

The present disclosure relates to methods and compositions comprising naturally occurring light absorbing molecules for preventing damages from light exposure. Specific embodiments of this disclosure include fluorescent proteins from Brachiostoma lanceolatum.

Claims

1. A method for protecting the state of the skin from light damage in a subject in need thereof, in which said method comprises topically administering a composition comprising a dermatologically acceptable carrier or a vehicle and a functional Brachiostoma light absorbing protein polypeptide having SEQ ID No:

13 formulated for topical administration.

3. The method of claim 1 wherein the Brachiostoma polypeptide is a derived from Brachiostoma floridae or Brachiostoma lanceolatum.

4. The method of claim 1 or 2 wherein said method comprises topically administering a composition comprising a dermatologically acceptable carrier or a vehicle and functional recombinant multimeric Brachiostoma light absorbing protein polypeptides formulated for topical administration comprising SEQ ID NO:

13 or SEQ ID NO;

14.

5. The method of claim 4 wherein the functional recombinant multimeric Brachiostoma light absorbing protein polypeptides formulated for topical administration comprises polypeptides that are tetramers, trimers or dimers.

6. The method of claim 5 wherein the functional recombinant Brachiostoma light absorbing protein polypeptides formulated for topical administration are tetramers.

7. The method of claims 1 or 2 wherein the recombinant multimeric protein is a heterotetrameric protein.

8. The method of anyone of claims 1, 2, or 3 wherein the method reduces UV-induced cellular damage.

2. A method for protecting the state of the skin from light damage in a subject in need thereof, in which said method comprises topically administering a composition comprising a dermatologically acceptable carrier or a vehicle and a functional Brachiostoma light absorbing protein polypeptide having SEQ ID No:

14 formulated for topical administration.