Fabrication of vascularized tissue using microfabricated two-dimensional molds
First Claim
1. A tissue lamina assembly comprising:
- a lower mold having an upper side defining lower mold microchannels;
an upper mold having a lower side defining upper mold microchannels that oppose the lower mold microchannels;
a semi-permeable membrane disposed between the lower and upper molds to separate the respective microchannels; and
cells cultured on at least one side of the semi-permeable membrane.
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Abstract
Methods and materials for making complex, living, vascularized tissues for organ and tissue replacement, especially complex and/or thick, structures, such as liver tissue is provided. Tissue lamina is made in a system comprising an apparatus having (a) a first mold or polymer scaffold, a semi-permeable membrane, and a second mold or polymer scaffold, wherein the semi-permeable membrane is disposed between the first and second molds or polymer scaffolds, wherein the first and second molds or polymer scaffolds have means defining microchannels positioned toward the semi-permeable membrane, wherein the first and second molds or polymer scaffolds are fastened together; and (b) animal cells. Methods for producing complex, three-dimensional tissues or organs from tissue lamina are also provided.
49 Citations
20 Claims
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1. A tissue lamina assembly comprising:
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a lower mold having an upper side defining lower mold microchannels; an upper mold having a lower side defining upper mold microchannels that oppose the lower mold microchannels; a semi-permeable membrane disposed between the lower and upper molds to separate the respective microchannels; and cells cultured on at least one side of the semi-permeable membrane. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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16. A lamina assembly capable of supplementing or replacing at least one organ function, comprising:
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a lower mold having an upper side defining a plurality of branched microchannels; an upper mold having a lower side defining at least one channel that opposes at least a portion of the branched microchannels; and a semi-permeable membrane disposed between the branched microchannels and the at least one channel. - View Dependent Claims (17, 18, 19, 20)
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Specification