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Microfluidic cartridge for processing and detecting nucleic acids

  • US 9,738,887 B2
  • Filed: 02/13/2013
  • Issued: 08/22/2017
  • Est. Priority Date: 02/13/2012
  • Status: Active Grant
First Claim
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1. A cartridge, configured to facilitate processing and detecting of a nucleic acid, comprising:

  • a first layer, defining a sample port, a reagent port, a fluid port, and a detection chamber;

    an elastomeric layer;

    an intermediate substrate coupled to the first layer, such that the elastomeric layer is situated between the intermediate substrate and the first layer, wherein the intermediate substrate defines a chamber with a corrugated surface directly opposing the first layer, wherein the corrugated surface defines a set of voids external to the chamber and accessible from a direction perpendicular to a broad surface of the first layer, and wherein at least a portion of the corrugated surface includes a set of openings that provide access to the elastomeric layer; and

    a fluidic pathway, wherein the fluidic pathway is fluidically coupled to the sample port, the reagent port, the fluid port, and the detection chamber.

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