Transgenic brassica event MON 88302 and methods of use thereof

• US 9,738,903 B2
• Filed: 07/16/2014
• Issued: 08/22/2017
• Est. Priority Date: 06/04/2010
• Status: Active Grant

• Current Assignee: Monsanto Technology LLC (Bayer AG)
• Original Assignee: Monsanto Technology LLC (Bayer AG)
• Inventors: Brown, Andrew J., Byrne, James F., Cole, Robert H., Crowley, James H., Miklos, John A., Ripley, Robert C., Seifert-Higgins, Simone, Xie, Jiali
• Primary Examiner(s): Kovalenko, Mykola V
• Application Number:
  US14/333,479
• Publication Number:
  US 20140373190A1
• Time in Patent Office: 1,133 Days
• Field of Search: None
• CPC Class Codes: A01N 57/20 containing acyclic or cycloaliphatic radicals
• CPC Class Codes: C12N 15/8275 Glyphosate

Abstract

The invention provides plants comprising transgenic event MON 88302 that exhibit tolerance to glyphosate herbicide. The invention also provides seeds, plant parts, cells, commodity products, and methods related to the event. The invention also provides DNA molecules that are unique to the event and were created by the insertion of transgenic DNA into the genome of a Brassica napus plant.

Claims

1. A method of producing a Brassica napus plant that tolerates application of glyphosate herbicide comprising:

a) crossing a glyphosate tolerant Brassica napus plant comprising event MON 88302, a representative sample of seed comprising said event having been deposited under ATCC Accession Number PTA-10955, with a second Brassica napus plant, thereby producing seed;

b) growing said seed to produce a plurality of progeny plants; and

c) selecting a progeny plant that comprises event MON 88302.

2. The method of claim 1, wherein said selecting a progeny plant comprises treating said progeny plants with glyphosate and selecting a progeny plant that is tolerant to glyphosate.

3. The method of claim 1, wherein said selecting a progeny plant comprises identifying a progeny plant comprising a DNA molecule having a nucleotide sequence selected from the group consisting of SEQ ID NO:

1, SEQ ID NO;

2, SEQ ID NO;

3, SEQ ID NO;

4, SEQ ID NO;

7, and SEQ ID NO;

8.

4. The method of claim 1, wherein said second Brassica napus plant lacks tolerance to glyphosate herbicide.

6. The method of claim 3, wherein said selecting a progeny plant comprises identifying a progeny plant comprising a DNA molecule having the nucleotide sequence of SEQ ID NO:

1.

7. The method of claim 3, wherein said selecting a progeny plant comprises identifying a progeny plant comprising a DNA molecule having the nucleotide sequence of SEQ ID NO:

2.

8. The method of claim 3, wherein said selecting a progeny plant comprises identifying a progeny plant comprising a DNA molecule having the nucleotide sequence of SEQ ID NO:

3.

9. The method of claim 3, wherein said selecting a progeny plant comprises identifying a progeny plant comprising a DNA molecule having the nucleotide sequence of SEQ ID NO:

4.

10. The method of claim 3, wherein said selecting a progeny plant comprises identifying a progeny plant comprising a DNA molecule having the nucleotide sequence of SEQ ID NO:

7.

11. The method of claim 3, wherein said selecting a progeny plant comprises identifying a progeny plant comprising a DNA molecule having the nucleotide sequence of SEQ ID NO:

8.

12. The method of claim 1, wherein said second Brassica napus plant comprises tolerance to glyphosate herbicide.

21. The method of claim 3, wherein said second Brassica napus plant comprises tolerance to glyphosate herbicide.

5. A method of producing a Brassica napus plant that tolerates application of glyphosate herbicide comprising:

a) selfing a glyphosate tolerant event Brassica napus plant comprising event MON 88302, a representative sample of seed comprising said event having been deposited under ATCC Accession Number PTA-10955, thereby producing seed;

b) growing said seed to produce a plurality of progeny plants; and

c) selecting a progeny plant that comprises event MON 88302.

13. The method of claim 5, wherein said selecting a progeny plant comprises treating said progeny plants with glyphosate and selecting a progeny plant that is tolerant to glyphosate.

14. The method of claim 5, wherein said selecting a progeny plant comprises identifying a progeny plant comprising a DNA molecule having a nucleotide sequence selected from the group consisting of SEQ ID NO:

1, SEQ ID NO;

2, SEQ ID NO;

3, SEQ ID NO;

4, SEQ ID NO;

7, and SEQ ID NO;

8.

15. The method of claim 5, wherein said selecting a progeny plant comprises identifying a progeny plant comprising a DNA molecule having the nucleotide sequence of SEQ ID NO:

1.

16. The method of claim 5, wherein said selecting a progeny plant comprises identifying a progeny plant comprising a DNA molecule having the nucleotide sequence of SEQ ID NO:

2.

17. The method of claim 5, wherein said selecting a progeny plant comprises identifying a progeny plant comprising a DNA molecule having the nucleotide sequence of SEQ ID NO:

3.

18. The method of claim 5, wherein said selecting a progeny plant comprises identifying a progeny plant comprising a DNA molecule having the nucleotide sequence of SEQ ID NO:

4.

19. The method of claim 5, wherein said selecting a progeny plant comprises identifying a progeny plant comprising a DNA molecule having the nucleotide sequence of SEQ ID NO:

7.

20. The method of claim 5, wherein said selecting a progeny plant comprises identifying a progeny plant comprising a DNA molecule having the nucleotide sequence of SEQ ID NO:

8.