Influenza treatment and/or characterization, human-adapted HA polypeptides; vaccines

• US 9,745,352 B2
• Filed: 07/25/2014
• Issued: 08/29/2017
• Est. Priority Date: 09/21/2010
• Status: Active Grant

• Current Assignee: Massachusetts Institute of Technology
• Original Assignee: Massachusetts Institute of Technology
• Inventors: Raman, Rahul, Koh, Xiaoying, Viswanathan, Karthik, Sasisekharan, Ram, Chandrasekaran, Aarthi
• Primary Examiner(s): Andres, Janet L
• Assistant Examiner(s): SNYDER, STUART
• Application Number:
  US14/341,285
• Publication Number:
  US 20150147329A1
• Time in Patent Office: 1,131 Days
• Field of Search: None
• CPC Class Codes: A61K 39/12 Viral antigens
• CPC Class Codes: A61K 39/145 Orthomyxoviridae, e.g. influenza virus
• CPC Class Codes: A61P 31/16 for influenza or rhinoviruses
• CPC Class Codes: A61P 37/04 Immunostimulants
• CPC Class Codes: C07K 14/005 from viruses
• CPC Class Codes: C07K 16/1018 Orthomyxoviridae, e.g. influenza virus
• CPC Class Codes: C12N 2760/16122 New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
• CPC Class Codes: C12N 2760/16133 Use of viral protein as therapeutic agent other than vaccine, e.g. apoptosis inducing or anti-inflammatory
• CPC Class Codes: C12N 2760/16134 Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
• CPC Class Codes: C12N 7/00 Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof preparing medicinal viral antigen or antibody compositions, e.g. virus vaccines, A61K39/00
• CPC Class Codes: G01N 2333/11 Orthomyxoviridae, e.g. influenza virus
• CPC Class Codes: G01N 33/56983 Viruses

Abstract

The present invention provides, among other things, methods, reagents, and systems for the treatment, detection, analysis, and/or characterization of influenza infections.

Claims

1. A monoclonal antibody that binds specifically to an HA polypeptide variant relative to that of reference HA from avian influenza strain A/Chicken/Pennsylvania/2004, wherein the HA polypeptide variant has an amino acid sequence that shows at least 90% overall sequence identity with a reference HA polypeptide of SEQ ID NO:

1, which HA polypeptide variant is characterized in that its amino acid sequence includes;

an amino acid residue (“

Residue 137”

) at a position corresponding to HA position 137 of SEQ ID NO;

1 that is arginine;

an amino acid residue (“

Residue 193”

) at a position corresponding to HA position 193 of SEQ ID NO;

1 that is threonine;

an amino acid residue (“

Residue 226”

) at a position corresponding to HA position 226 of SEQ ID NO;

1 that is leucine; and

an amino acid residue (“

Residue 228”

) at a position corresponding to HA position 228 of SEQ ID NO;

1 that is serine.

2. A method of using the antibody of claim 1 to detect the HA polypeptide in a sample.

3. The method of claim 2, wherein the sample is an environmental sample.

4. The method of claim 3, wherein the environmental sample is or comprises one or more of soil, water and flora.

5. The method of claim 2, wherein the sample is a pathological sample.

6. The method of claim 5, wherein the pathological sample is or comprises blood, serum, plasma, peripheral blood mononuclear cells, peripheral blood lymphocytes (PBMC/PBL), sputum, urine, feces, throat swabs, dermal lesion swabs, cerebrospinal fluids, cervical smears, pus samples, food matrices, and tissues.