Synthetic surfaces for culturing stem cell derived cardiomyocytes

• US 9,745,550 B2
• Filed: 01/25/2016
• Issued: 08/29/2017
• Est. Priority Date: 01/30/2008
• Status: Expired due to Fees

• Current Assignee: Asterias Biotherapeutics Incorporated (Lineage Cell Therapeutics, Inc.)
• Original Assignee: Asterias Biotherapeutics Incorporated (Lineage Cell Therapeutics, Inc.)
• Inventors: Shogbon, Christopher Bankole, Zhou, Yue, Brandenberger, Ralph
• Primary Examiner(s): BERTOGLIO, VALARIE E
• Application Number:
  US15/005,397
• Publication Number:
  US 20160137983A1
• Time in Patent Office: 582 Days
• Field of Search: 435395
• CPC Class Codes: C12N 2500/90 Serum-free medium, which may still contain naturally-sourced components
• CPC Class Codes: C12N 2506/02 from embryonic cells
• CPC Class Codes: C12N 2533/30 Synthetic polymers thermoreactive polymers, e.g. PNIPAm, C12N2539/10
• CPC Class Codes: C12N 5/0068 General culture methods using substrates for specific animal cell type C12N5/06
• CPC Class Codes: C12N 5/0657 Cardiomyocytes; Heart cells

Abstract

Synthetic surfaces suitable for culturing stem cell derived cardiomyocytes contain acrylate polymers formed from one or more acrylate monomers. The acrylate surfaces, in many cases, are suitable for culturing stem cell derived cardiomyocytes in chemically defined media.

Claims

1. A method for culturing pluripotent stem-cell derived cardiomyocytes, comprising:

depositing a suspension comprising the pluripotent stem-cell derived cardiomyocytes on a polymer material, and culturing the deposited pluripotent stem-cell derived cardiomyocytes in a cell culture medium, wherein the polymer material comprises a diacrylate or a dimethacrylate.

2. The method of claim 1, wherein the diacrylate is poly(ethylene glycol) diacrylate.

3. The method of claim 1, wherein the dimethacrylate is glycerol dimethacrylate.

4. The method of claim 1, wherein the dimethacrylate is 1,4-butanediol dimethacrylate.

5. The method of claim 1 wherein the pluripotent stem-cell derived cardiomyocytes are human cells.

6. The method of claim 1, wherein the pluripotent stem-cell derived cardiomyocytes are human embryonic stem-cell derived cardiomyocytes.

7. The method of claim 1, wherein the cell culture medium is a chemically defined medium.