Real-time analytical methods and systems
First Claim
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1. A method of monitoring passage of a reaction component across a portion of an amphipathic aggregate, the method comprising:
- a) immobilizing a single amphipathic aggregate at a reaction site, wherein at least one molecule is imbedded in the single amphipathic aggregate and wherein the single amphipathic aggregate comprises an aggregate-confined reaction volume localized within a micelle, within a liposome, or within a structural confinement covered by a lipid bilayer;
b) exposing the single amphipathic aggregate to a reaction mixture comprising a labeled reaction component, wherein the labeled reaction component binds the imbedded molecule, wherein the binding results in the labeled molecule being transported into the aggregate-confined reaction volume, and wherein the labeled reaction component comprises a detectable label that emits a signal when present in the aggregate-confined reaction volume; and
c) detecting the signal at the reaction site, wherein the signal at the reaction site is indicative that the labeled reaction component has passed into the aggregate-confined reaction volume, thereby monitoring the passage of the labeled reaction component across a portion of the amphipathic aggregate.
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Abstract
The present invention is generally directed to compositions, methods, and systems for performing single-molecule, real-time analysis of a variety of different biological reactions, and for determining various characteristics of the different biological reactions. The ability to analyze such reactions provides an opportunity to study those reactions as well as to potentially identify factors and/or approaches for impacting such reactions, e.g., to stimulate, enhance, or inhibit such reactions.
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Citations
15 Claims
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1. A method of monitoring passage of a reaction component across a portion of an amphipathic aggregate, the method comprising:
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a) immobilizing a single amphipathic aggregate at a reaction site, wherein at least one molecule is imbedded in the single amphipathic aggregate and wherein the single amphipathic aggregate comprises an aggregate-confined reaction volume localized within a micelle, within a liposome, or within a structural confinement covered by a lipid bilayer; b) exposing the single amphipathic aggregate to a reaction mixture comprising a labeled reaction component, wherein the labeled reaction component binds the imbedded molecule, wherein the binding results in the labeled molecule being transported into the aggregate-confined reaction volume, and wherein the labeled reaction component comprises a detectable label that emits a signal when present in the aggregate-confined reaction volume; and c) detecting the signal at the reaction site, wherein the signal at the reaction site is indicative that the labeled reaction component has passed into the aggregate-confined reaction volume, thereby monitoring the passage of the labeled reaction component across a portion of the amphipathic aggregate. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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Specification