Process for the production of a hybridoma and antibody obtained therefrom, able to recognize more than one vitamin D metabolite

• US 9,746,483 B2
• Filed: 05/11/2015
• Issued: 08/29/2017
• Est. Priority Date: 10/27/2009
• Status: Active Grant

• Current Assignee: Diasource Immunoassays S.A. (BioVendor - Laboratorní medicína as)
• Original Assignee: Diasource Immunoassays S.A. (BioVendor - Laboratorní medicína as)
• Inventors: Anciaux, Michel, Mathieu, Fabienne, Lin, Frederic, Poncelet, Martin
• Primary Examiner(s): YAKOVLEVA, GALINA M
• Application Number:
  US14/708,594
• Publication Number:
  US 20150346224A1
• Time in Patent Office: 841 Days
• CPC Class Codes: C07K 16/26 against hormones ; against hormone releasing or inhibiting factors
• CPC Class Codes: C07K 16/44 against material not provided for elsewhere , e.g. haptens, metals, DNA, RNA, amino acids
• CPC Class Codes: C07K 2317/24 containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
• CPC Class Codes: C07K 2317/33 Crossreactivity, e.g. for species or epitope, or lack of said crossreactivity
• CPC Class Codes: G01N 33/577 involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens; G01N33/53 - G01N33/576 take precedence
• CPC Class Codes: G01N 33/82 involving vitamins or their receptors
• CPC Class Codes: Y10T 436/203332 Hydroxyl containing

Abstract

The invention concerns a process for the production of a hybridoma, and of a monoclonal antibody or fragments thereof able to recognize 25-hydroxyvitamin D₂ and 25-hydroxyvitamin D_3.

Claims

1. A hybridoma suitable for the production of a monoclonal antibody able to recognize 25-hydroxyvitamin D₂ and 25-hydroxyvitamin D₃, wherein the hydridoma is selected from the group consisting of the hybridomas deposited in the BCCM/LMBP under deposit numbers LMBP 7011CB, LMBP 7012CB, LMBP 7013CB, LMBP 7204CB and LMBP 7205CB.

2. A monoclonal antibody, produced from a hybridoma selected from the group consisting of the hybridomas deposited in the BCCM/LMBP under deposit numbers LMBP 7011CB, LMBP 7012CB, LMBP 7013CB, LMBP 7204CB and LMBP 7205CB.