Methods and compositions for improving the appearance of skin

• US 9,750,682 B2
• Filed: 07/30/2015
• Issued: 09/05/2017
• Est. Priority Date: 07/30/2015
• Status: Active Grant

• Current Assignee: ELC Management LLC (The Estee Lauder Companies Inc.)
• Original Assignee: ELC Management LLC (The Estee Lauder Companies Inc.)
• Inventors: Pernodet, Nadine, Collins, Donald
• Primary Examiner(s): Mi, Qiuwen
• Application Number:
  US14/813,212
• Publication Number:
  US 20170027854A1
• Time in Patent Office: 768 Days
• Field of Search: None
• CPC Class Codes: A61K 8/64 Proteins; Peptides; Derivatives or degradation products thereof
• CPC Class Codes: A61K 8/66 Enzymes
• CPC Class Codes: A61K 8/9711 Phaeophycota or Phaeophyta [brown algae], e.g. Fucus
• CPC Class Codes: A61K 8/9728 Fungi, e.g. yeasts
• CPC Class Codes: A61K 8/9794 Liliopsida [monocotyledons]
• CPC Class Codes: A61Q 19/08 Anti-ageing preparations

Abstract

A method for stimulating collagen synthesis in aging skin cells in need of treatment by stimulating SIRT1, SIRT3, and SIRT6 and a method for preparing a topical composition for stimulating SIRT1, SIRT3, and SIRT6 in skin cells.

Claims

1. A method for stimulating collagen synthesis in skin cells in need of such treatment by topically applying a composition comprising at least one extract from Laminaria genus, at least one extract from the Narcissus genus, and at least one peptide of the formula (I):

R₁-(AA)_n-X₁-X₂-X₃-X₄-X₅-X₆-(AA)_p-R₂, in which

X₁ is glycine or threonine or histidine;

X₂ is alanine or glutamine or glycine;

X₃ is glycine or asparagine or serine;

X₄ is valine or isoleucine or leucine;

X₅ is serine or aspartic acid or phenylalanine;

X₆ is alanine or glutamic acid or lysine;

wherein

when X₁ is glycine, then X₂ is alanine and X₃ is glycine;

when X₁ is threonine, then X₃ is asparagine;

when X₁ is histidine, then X₂ is glycine;

AA represents any amino acid and n and p are integers between 0 and 2;

R₁ represents the primary amino function of the N-terminal amino acid, free or substituted by an acyl type group having either an alkyl chain from C_1-30, saturated or unsaturated, that may be an acetyl group, or an aromatic group that may be chosen from among a benzoyl, tosyl or benzyloxycarbonyl type group; and

R₂ represents the hydroxyl group of the carboxyl function of the C-terminal amino acid, free or substituted by a group that may be chosen from among an alkyl chain from C1-30, or an NH₂, NHY or NYY group with Y representing an alkyl chain from C1-4 wherein the extract from the Laminaria genus is present at 0.001 to 1%, the extract from Narcissus genus is present at 0.001 to 1.5% and the peptide is present from 0.0001 to 1%, with all percentages by weight of the total composition.

2. The method of claim 1 wherein the stimulation of collagen synthesis causes a reduction in skin laxity, a reduction in the appearance of lines or wrinkles, or both.

3. The method of claim 1 wherein the topical composition is applied once or twice per day.

4. The method of claim 1 wherein the topical composition is applied before a period of sustained rest.

5. The method of claim 1 wherein the extract from the Laminaria genus stimulates SIRT3activity in skin cells.

6. The method of claim 5 wherein the extract from the Laminaria genus is Laminaria saccharina.

7. The method of claim 1 wherein the extract from the Narcissus genus stimulates SIRT1 activity in skin cells.

8. The method of claim 7 wherein the extract from the Narcissus genus is Narcissus tazetta extract from the bulb in its dormant phase of growth.

9. The method of claim 1 wherein the peptide is selected from the group consisting of:

10. The method of claim 9 wherein the peptide is of the following formula:

11. The method of claim 1 wherein the peptide incorporated into the composition is in the form of yeast extract with the peptide being a component of the extract.

12. The method of claim 1 wherein the extract from the Laminaria genus is Laminaria digitata.

13. The method of claim 1 wherein the extract from the Laminaria genus is one or more of Laminaria digitata or Laminaria saccharina and the extract from Narcissus is Narcissus tazetta.

14. The method of claim 1 wherein the skin cells are dermal fibroblasts.