Method of preparing autologous cells and methods of use for therapy
First Claim
1. A method of preparing a therapeutic cell population expanded in autologous medium comprising:
- harvesting blood from a donor in two visits separated between one and three weeks;
harvesting bone marrow cells from the donor;
forming a culture medium from said blood that contains no animal serum and comprises 10% autologous serum obtained from the donor; and
expanding a population of mesenchymal stem cells from the bone marrow cells in the culture medium.
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Abstract
A method for expanding mesenchymal cells derived from autologous bone marrow in autologous culture medium which can be used in a clinical setting, and a business method for performing such expansions in the future as a service for patients. A method for expanding mesenchymal cells derived from autologous bone marrow in autologous culture medium including a diagnostic kit for the autologous cell therapy to determine whether a patient will respond to the autologous cell therapy for treatment of a disease, in which said kit comprising a system for detecting gene and protein expression comprising at least two isolated DNA molecules wherein each isolated DNA molecule detects expression of a gene that is differentially expressed in the tissue of the patient that is intended to be the source of the autologous cell therapy.
31 Citations
4 Claims
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1. A method of preparing a therapeutic cell population expanded in autologous medium comprising:
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harvesting blood from a donor in two visits separated between one and three weeks; harvesting bone marrow cells from the donor; forming a culture medium from said blood that contains no animal serum and comprises 10% autologous serum obtained from the donor; and expanding a population of mesenchymal stem cells from the bone marrow cells in the culture medium. - View Dependent Claims (2, 3, 4)
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Specification