Methods of producing isoprene and a co-product
First Claim
1. Recombinant host cells comprising (a) a heterologous nucleic acid encoding an isoprene synthase polypeptide, (b) a nucleic acid encoding an isopentenyl-diphosphate delta-isomerase (IDI) polypeptide, (c) nucleic acid(s) encoding a 1-Deoxyxylulose-5-phosphate synthase (DXS) polypeptide and/or one or more mevalonate (MVA) pathway polypeptides and (d) heterologous nucleic acid(s) encoding (i) one or more polypeptide involved in ethanol fermentation, wherein the polypeptide involved in ethanol fermentation is an alcohol dehydrogenase from yeast or bacteria, or (ii) a pyruvate decarboxylase (pdc) polypeptide,wherein said cells co-produce isoprene and ethanol under oxygen-limited culture conditions, wherein the oxygen transfer rate (OTR) is less than the oxygen uptake rate (OUR).
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Abstract
The invention features methods producing isoprene and a co-product, such as ethanol, 1,3-propanediol, or hydrogen from cultured cells. The invention also provides compositions that include these cultured cells. The invention provides compositions comprising isoprene and ethanol, isoprene and 1,3-propanediol, and isoprene and hydrogen. Additionally, the invention provides methods of co-producing isoprene and ethanol, isoprene and 1,3-propanediol, and isoprene and hydrogen by culturing cells under conditions suitable for co-production of isoprene and ethanol, isoprene and 1,3-propanediol, and isoprene and hydrogen.
22 Citations
32 Claims
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1. Recombinant host cells comprising (a) a heterologous nucleic acid encoding an isoprene synthase polypeptide, (b) a nucleic acid encoding an isopentenyl-diphosphate delta-isomerase (IDI) polypeptide, (c) nucleic acid(s) encoding a 1-Deoxyxylulose-5-phosphate synthase (DXS) polypeptide and/or one or more mevalonate (MVA) pathway polypeptides and (d) heterologous nucleic acid(s) encoding (i) one or more polypeptide involved in ethanol fermentation, wherein the polypeptide involved in ethanol fermentation is an alcohol dehydrogenase from yeast or bacteria, or (ii) a pyruvate decarboxylase (pdc) polypeptide,
wherein said cells co-produce isoprene and ethanol under oxygen-limited culture conditions, wherein the oxygen transfer rate (OTR) is less than the oxygen uptake rate (OUR).
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18. A method of co-producing isoprene and ethanol, the method comprising:
- (a) culturing recombinant host cells capable of co-producing isoprene and ethanol under oxygen limited culture conditions wherein, the oxygen transfer rate (OTR) is less than the oxygen uptake rate (OUR), and wherein the cells comprise (a) a heterologous nucleic acid encoding an isoprene synthase polypeptide (b) a nucleic acid encoding an isopentenyl-diphosphate delta-isomerase (IDI) polypeptide, (c) nucleic acid(s) encoding a 1-Deoxyxylulose-5-phosphate synthase (DXS) polypeptide and/or one or more mevalonate (MVA) pathway polypeptides and (d) heterologous nucleic acid(s) encoding (i) one or more polypeptide involved in ethanol fermentation, wherein the polypeptide involved in ethanol fermentation is an alcohol dehydrogenase from yeast or bacteria, or (ii) a pyruvate decarboxylase (pdc) polypeptide; and
(b) co-producing isoprene and ethanol. - View Dependent Claims (19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32)
- (a) culturing recombinant host cells capable of co-producing isoprene and ethanol under oxygen limited culture conditions wherein, the oxygen transfer rate (OTR) is less than the oxygen uptake rate (OUR), and wherein the cells comprise (a) a heterologous nucleic acid encoding an isoprene synthase polypeptide (b) a nucleic acid encoding an isopentenyl-diphosphate delta-isomerase (IDI) polypeptide, (c) nucleic acid(s) encoding a 1-Deoxyxylulose-5-phosphate synthase (DXS) polypeptide and/or one or more mevalonate (MVA) pathway polypeptides and (d) heterologous nucleic acid(s) encoding (i) one or more polypeptide involved in ethanol fermentation, wherein the polypeptide involved in ethanol fermentation is an alcohol dehydrogenase from yeast or bacteria, or (ii) a pyruvate decarboxylase (pdc) polypeptide; and
Specification