Method for pretreating specimen and method for assaying biological substance
First Claim
1. A biologically-relevant substance assay device, comprising:
- (a) a specimen holding portion in which a specimen is held;
(b) a first holding portion in which trapping particles for trapping a biologically-relevant substance from the specimen are held;
(c) a second holding portion in which a reagent for detecting the biologically-relevant substance is held;
(d) a dispensing mechanism for dispensing the specimen or the biologically-relevant substance; and
(e) a light-irradiating mechanism for irradiating an irradiating light for letting the biologically-relevant substance fluoresce, and a detecting mechanism for receiving the fluorescence from the biologically-relevant substance to detect the biologically-relevant substance,wherein the light irradiating mechanism includes a light-irradiating optical fiber irradiating the irradiating light downwardly from a nozzle unit of the dispensing mechanism, the light-irradiating optical fiber is extended inside of the nozzle unit of the dispensing mechanism along the nozzle unit.
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Abstract
This invention relates to an assay device and a method for pretreating a specimen containing a biologically-relevant substance and then assaying the biologically-relevant substance. Biologically-relevant substances used in the assay method include microorganisms, cells, viruses, nucleic acids, polysaccharides, proteins (including antigens, antibodies, chromoproteins, and enzymes), peptides, nucleic acids, and small molecules. This pretreatment method removes contaminants from the biologically-relevant substances using supports such as magnetic particles, gels, resins, membranes, and solid-phased reagents. Therefore, in the assay method the following steps are generally carried out (i) a pretreatment step of treating a specimen using one or more first supports and then one or more second supports, and (ii) the assay step carried out after the pretreatment step. In particular, the pretreatment method reduces false positives and false negatives in the assay.
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Citations
13 Claims
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1. A biologically-relevant substance assay device, comprising:
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(a) a specimen holding portion in which a specimen is held; (b) a first holding portion in which trapping particles for trapping a biologically-relevant substance from the specimen are held; (c) a second holding portion in which a reagent for detecting the biologically-relevant substance is held; (d) a dispensing mechanism for dispensing the specimen or the biologically-relevant substance; and (e) a light-irradiating mechanism for irradiating an irradiating light for letting the biologically-relevant substance fluoresce, and a detecting mechanism for receiving the fluorescence from the biologically-relevant substance to detect the biologically-relevant substance, wherein the light irradiating mechanism includes a light-irradiating optical fiber irradiating the irradiating light downwardly from a nozzle unit of the dispensing mechanism, the light-irradiating optical fiber is extended inside of the nozzle unit of the dispensing mechanism along the nozzle unit. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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Specification