Process for making biosensor

• US 9,755,390 B2
• Filed: 04/17/2013
• Issued: 09/05/2017
• Est. Priority Date: 04/18/2012
• Status: Active Grant

• Current Assignee: Noviosense B.V.
• Original Assignee: Noviosense B.V.
• Inventors: Hanssen, Johannes Hendrikus Leonardus, Wilson, Christopher
• Primary Examiner(s): Tugbang, A. Dexter
• Application Number:
  US14/394,873
• Publication Number:
  US 20150068028A1
• Time in Patent Office: 1,602 Days
• CPC Class Codes: A61B 2560/04 Constructional details of apparatus
• CPC Class Codes: A61B 2562/0215 Silver or silver chloride containing
• CPC Class Codes: A61B 2562/12 Manufacturing methods specially adapted for producing sensors for in-vivo measurements
• CPC Class Codes: A61B 5/0031 Implanted circuitry
• CPC Class Codes: A61B 5/14532 for measuring glucose, e.g. by tissue impedance measurement
• CPC Class Codes: A61B 5/14865 invasive, e.g. introduced into the body by a catheter or needle or using implanted sensors
• CPC Class Codes: A61B 5/4839 combined with drug delivery
• CPC Class Codes: A61N 1/0563 specially adapted for defibrillation or cardioversion
• CPC Class Codes: C12Q 1/006 for glucose
• CPC Class Codes: G01N 2333/904 acting on CHOH groups as donors, e.g. glucose oxidase, lactate dehydrogenase (1.1)
• CPC Class Codes: G01N 33/487 of liquid biological material
• CPC Class Codes: G01N 33/543 with an insoluble carrier for immobilising immunochemicals
• CPC Class Codes: G01N 33/54306 Solid-phase reaction mechanisms
• CPC Class Codes: G01N 33/5438 Electrodes
• CPC Class Codes: G01N 33/54393 Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
• CPC Class Codes: H01M 4/137 Electrodes based on electro-active polymers
• CPC Class Codes: H01M 4/1399 of electrodes based on electro-active polymers
• CPC Class Codes: H01R 43/033 for wrapping or unwrapping wire connections
• CPC Class Codes: H01R 43/28 for wire processing before connecting to contact members, not provided for in groups H01R43/02 - H01R43/26
• CPC Class Codes: Y02E 60/10 Energy storage using batteries
• CPC Class Codes: Y10T 29/49018 : with other electrical component
• CPC Class Codes: Y10T 29/49194 : Assembling elongated conductors, e.g., splicing, etc.

Abstract

A process for making a biosensor comprising a hollow coil having wires coiled in parallel and an electronic circuit component connected to the coil, the process including: 1) providing a mandrel on which wires including at least a first wire, a second wire and a third wire are wound in parallel, 2a) immersing the mandrel in a first buffer solution comprising a first bioreceptor, a first monomer and optional additives, 2b) arranging the wires such that the first wire may be used as a working electrode, the second wire may be used as a counter electrode and the third wire may be used as a reference electrode of a three electrode electrochemical cell used in an electropolymerization process, 3) passing electric current through the first wire to form a first biocompatible coating of a first polymer polymerized from the first monomer comprising the first bioreceptor on the first wire, 4) removing the coil from the mandrel, 5) connecting the wires to their respective points of the electronic circuit component such that the first wire may be used as a working electrode, the second wire may be used as a counter electrode and the third wire may be used as a reference electrode and wherein the electronic circuit component is configured such that it can generate an input signal for a wireless receiver based upon the activity of the bioreceptor and wirelessly send the input signal to the wireless receiver.

Claims

1. A process for making a biosensor comprising a hollow coil comprising wires coiled in parallel and an electronic circuit component connected to the coil, the process comprising:

1) providing a mandrel on which wires including at least a first wire, a second wire and a third wire are wound in parallel,

2a) immersing the mandrel in a first buffer solution comprising a first bioreceptor and a first monomer,

2b) arranging the wires such that the second wire is used as a working electrode, the first wire is used as a counter electrode and the third wire is used as a reference electrode of a three electrode electrochemical cell used in an electropolymerisation process,

3) passing electric current through the second wire to form a first biocompatible coating of a first polymer polymerized from the first monomer comprising the first bioreceptor on the second wire,

4) removing the coil from the mandrel,

5) connecting the wires to their respective points of the electronic circuit component such that the second wire may be used, as a second working electrode, the first wire is used as a second counter electrode and the third wire is used as a second reference electrode,

wherein the electronic circuit component is configured such that the electronic circuit component can generate an input signal for a wireless receiver based upon the activity of the bioreceptor and wirelessly send the input signal to the wireless receiver,

wherein the first monomer is a five-membered heterocycle of formula (I)

2. The process according to claim 1, wherein the wires include a further wire and the process further comprises the steps of:

2a′

) immersing the mandrel in a second buffer solution comprising a second bioreceptor and a second monomer, wherein the second bioreceptor is different from the first bioreceptor,

2b′

) arranging the wires such that the further wire may be used as a third working electrode, the first wire is used as a third counter electrode and the third wire is used as a third reference electrode of a second three electrode electrochemical cell used in an electropolymerisation process,

3′

) passing electric current through the further wire to form a second biocompatible coating of a second polymer polymerized from the second monomer comprising the second bioreceptor on the further wire and

wherein step

     5) comprises the step of connecting the further wire to the electronic circuit component such that the further wire may be used as a fourth working electrode.

3. The process according to claim 2, wherein the process further comprises the step of providing a biocompatible resin capping on the electric circuit component after step 5).

4. The process according to claim 3, wherein the wires further include a fourth wire coated with an insulating layer and wherein step 5) comprises the step of connecting the fourth wire to the electronic circuit component such that the fourth wire is used as an antenna.

5. The process according to claim 4, wherein the wires further include a fifth wire coated with a fifth wire insulating layer which is used as a spacer.

6. The process according to claim 5, further comprising the step of cutting the coil into a length of 5-50 mm between steps 4) and 5).

7. The process according to claim 6, further comprising the step of providing a top coating of a biocompatible material on the biosensor after step 5).

8. The process according to claim 7, wherein the sensor has a diameter of 0.1 to 3 mm.

9. The process according to claim 8, wherein the first coiled wire has a Pt surface.

10. The process according to claim 9, wherein the third coiled wire is a silver/silver chloride reference electrode, wherein the bioreceptor is an oxidoreductase of the enzyme commission groups EC 1.X.3 where X=1-17, and wherein the bioreceptor is glucose oxidase, lactate dehydrogenase, pyruvate dehydrogenase or pyruvate oxidase.

11. The process according to claim 1, wherein the process further comprises the step of providing a biocompatible resin capping on the electric circuit component after step 5).

12. The process according to claim 1, wherein the wires further include a fourth wire coated with an insulating layer and wherein step 5) comprises the step of connecting the fourth wire to the electronic circuit component such that the fourth wire is used as an antenna.

13. The process according to claim 12, wherein the wires further include a fifth wire coated with a fifth wire insulating layer which is used as a spacer.

14. The process according to claim 1, further comprising the step of cutting the coil into a length of 5-50 mm between steps 4) and 5).

15. The process according to claim 1, further comprising the step of providing a top coating of a biocompatible material on the biosensor after step 5).

16. The process according to claim 1, wherein the sensor has a diameter of 0.1 to 3 mm.

17. The process according to claim 1, wherein the first coiled wire has a Pt surface.

18. The process according to claim 1, wherein the third coiled wire is a silver/silver chloride reference electrode.

19. The process according to claim 1, wherein the bioreceptor is an oxidoreductase of the enzyme commission groups EC 1.X.3 where X=1-17.

20. The process according to claim 1, wherein the bioreceptor is glucose oxidase, lactate dehydrogenase, pyruvate dehydrogenase or pyruvate oxidase.