Collagen scaffolds

• US 9,757,495 B2
• Filed: 01/31/2014
• Issued: 09/12/2017
• Est. Priority Date: 02/01/2013
• Status: Active Grant

• Current Assignee: Children's Medical Center Corporation
• Original Assignee: Children's Medical Center Corporation
• Inventors: Murray, Martha M.
• Primary Examiner(s): GUDIBANDE, SATYANARAYAN R
• Application Number:
  US14/765,064
• Publication Number:
  US 20150367030A1
• Time in Patent Office: 1,320 Days
• Field of Search: None
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: A61L 2430/06 for cartilage reconstruction, e.g. meniscus
• CPC Class Codes: A61L 2430/10 for reconstruction of tendons or ligaments
• CPC Class Codes: A61L 27/24 Collagen
• CPC Class Codes: A61L 27/3633 Extracellular matrix [ECM]
• CPC Class Codes: A61L 27/3691 characterised by physical conditions of the treatment, e.g. applying a compressive force to the composition, pressure cycles, ultrasonic/sonication or microwave treatment, lyophilisation
• CPC Class Codes: A61L 27/56 Porous materials, e.g. foams or sponges
• CPC Class Codes: C07K 14/78 Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
• CPC Class Codes: C12P 21/00 Preparation of peptides or proteins single cell protein C12N1/00

Abstract

Methods for preparing and using collagen extracts and collagen scaffolds are provided. Additionally methods and related kits for the repair of articular tissue using the collagen material are provided.

Claims

1. A method for preparing a neutralized collagen slurry comprising,

lyophilizing a source tissue;

cutting the source tissue into small pieces while keeping the tissue frozen,

performing an enzyme digestion of the small pieces of source tissue to produce a collagen extract,

lyophilizing the collagen extract to form a lyophilized collagen extract,

reconstituting the lyophilized collagen extract to form a collagen slurry having a collagen concentration of greater than 10 mg/ml, and

neutralizing the collagen slurry to produce a neutralized collagen slurry.

2. The method of claim 1, wherein the collagen extract is treated with hyperosmotic solutions to remove cellular debris.

3. The method of claim 1, wherein the collagen extract is treated with detergents to remove cellular debris.

4. The method of claim 2, further comprising freeze drying a collagen extract prior to the neutralization step.

5. The method of claim 1, further comprising adding calcium to the collagen extract.

6. The method of claim 1, wherein the collagen slurry is added to a mold prior to neutralization.

7. The method of claim 6, wherein the mold is cylindrical.

8. The method of claim 1, further comprising subjecting the neutralized collagen slurry to an elevated temperature such that the neutralized collagen slurry forms a collagen scaffold comprised of self-assembled collagen fibers.

9. The method of claim 1, wherein the source tissue is obtained from a knee.

10. The method of claim 1, wherein the source tissue is obtained from a bovine knee.