Methods and compositions for somatic cell proliferation and viability

• US 9,758,763 B2
• Filed: 05/27/2014
• Issued: 09/12/2017
• Est. Priority Date: 05/29/2013
• Status: Active Grant

• Current Assignee: Regents of the University of California (University of California)
• Original Assignee: Regents of the University of California (University of California)
• Inventors: Conboy, Irina M., Conboy, Michael J., Yousef, Hanadie, Schaffer, David V.
• Primary Examiner(s): Bowers, Erin M
• Application Number:
  US14/288,117
• Publication Number:
  US 20140356947A1
• Time in Patent Office: 1,204 Days
• Field of Search: None
• CPC Class Codes: C12N 2501/91 Heparin
• CPC Class Codes: C12N 2502/02 embryonic cells
• CPC Class Codes: C12N 5/0619 Neurons
• CPC Class Codes: C12N 5/0659 Satellite cells

Abstract

Methods and compositions for somatic cell proliferation as well as increasing viability of somatic cells are provided. The compositions include heparin binding protein isolated from a medium conditioned by growth of pluripotent stem cells, such as, human embryonic stem cells, human embryonic carcinoma cells. The methods include contacting a somatic cell with a heparin binding protein composition for a sufficient period of time to provide for enhanced proliferation and/or viability of the somatic cell as compared to the absence of the heparin binding protein composition.

Claims

1. A method for enhancing proliferation of a muscle cell, the method comprising:

isolating all heparin binding proteins from a medium conditioned by growth of human embryonic stem cells or human embryonic carcinoma cells, wherein the medium is essentially free of human embryonic stem cells or human embryonic carcinoma cells and wherein the isolating comprises contacting the medium with a solid support conjugated to heparin and eluting the heparin binding proteins bound to the solid support;

contacting a muscle cell with a composition comprising all of the heparin binding proteins isolated from the medium conditioned by growth of human embryonic stem cells or human embryonic carcinoma cells,

wherein the contacting is for a period of time sufficient to provide for enhanced proliferation of the muscle cell compared to the absence of the heparin binding proteins.

2. The method of claim 1, wherein the muscle cell is cell of an aged subject.

3. The method of claim 1, wherein the muscle cell is an injured cell.

4. The method of claim 3, wherein the injury is caused by a disease.

5. The method of claim 1, wherein the muscle cell is a diseased cell.

6. The method of claim 1, wherein the muscle cell is a skeletal muscle cell.

7. The method of claim 6, wherein the skeletal muscle cell is a myoblast.

8. The method of claim 1, wherein the muscle cell is an activated satellite cell.

9. The method of claim 1, wherein the heparin binding proteins are isolated from a medium conditioned by growth of human embryonic stem cells.

10. The method of claim 2, wherein the muscle cell is a skeletal muscle cell.

11. The method of claim 10, wherein the skeletal muscle cell is a myoblast.

12. The method of claim 2, wherein the muscle cell is an activated satellite cell.

13. The method of claim 3, wherein the muscle cell is a skeletal muscle cell.