Genetically engineered and acid-resistant yeast cell with enhanced activity of radiation sensitivity complementing kinase and method of producing lactate by using the yeast cell
First Claim
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1. A recombinant acid-resistant yeast cell comprising increased radiation sensitivity complementing kinase (RCK) activity as compared to the RCK activity of a parent cell of the recombinant yeast cell, wherein the yeast cell comprises a genetic modification that increases the RCK activity,wherein an activity of JEN1 is increased in the recombinant yeast cell as compared with that of a parent cell of the recombinant acid-resistant yeast cell;
- wherein the recombinant acid-resistant yeast cell further comprises a polynucleotide encoding a lactate dehydrogenase; and
deletion or disruption of;
a gene encoding a polypeptide that converts pyruvate to acetaldehyde;
a gene encoding a polypeptide that converts lactate to pyruvate;
a gene encoding a polypeptide that converts dihydroxyacetone phosphate (DHAP) to glycerol-3-phosphate;
a gene encoding NDE1 or NDE2, or both a gene encoding NDE1 and a gene encoding NDE2;
a gene encoding a mitochondrial pyruvate carrier (MPC);
a gene encoding a polypeptide that converts pyruvate to oxaloacetate;
a gene encoding a polypeptide that converts acetaldehyde to ethanol; and
a gene encoding Fps1.
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Abstract
Provided is an acid-resistant yeast cell that is genetically engineered to enhance activity of a radiation sensitivity complementing kinase, and a method of producing lactate by using the yeast cell.
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Citations
17 Claims
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1. A recombinant acid-resistant yeast cell comprising increased radiation sensitivity complementing kinase (RCK) activity as compared to the RCK activity of a parent cell of the recombinant yeast cell, wherein the yeast cell comprises a genetic modification that increases the RCK activity,
wherein an activity of JEN1 is increased in the recombinant yeast cell as compared with that of a parent cell of the recombinant acid-resistant yeast cell; -
wherein the recombinant acid-resistant yeast cell further comprises a polynucleotide encoding a lactate dehydrogenase; and
deletion or disruption of;a gene encoding a polypeptide that converts pyruvate to acetaldehyde; a gene encoding a polypeptide that converts lactate to pyruvate; a gene encoding a polypeptide that converts dihydroxyacetone phosphate (DHAP) to glycerol-3-phosphate; a gene encoding NDE1 or NDE2, or both a gene encoding NDE1 and a gene encoding NDE2; a gene encoding a mitochondrial pyruvate carrier (MPC); a gene encoding a polypeptide that converts pyruvate to oxaloacetate; a gene encoding a polypeptide that converts acetaldehyde to ethanol; and a gene encoding Fps1. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
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Specification