×

Reagents and methods of PCR

  • US 9,758,813 B2
  • Filed: 05/18/2015
  • Issued: 09/12/2017
  • Est. Priority Date: 03/12/2009
  • Status: Active Grant
First Claim
Patent Images

1. A reaction mixture for a primer-dependent DNA amplification reaction including primer extension by a DNA polymerase for amplifying at least one DNA target sequence, said reaction mixture comprising:

  • (a) at least one primer pair;

    (b) a DNA polymerase;

    (c) dNTPs; and

    (d) at least one double-stranded oligonucleotide additive;

    (i) comprising two separate strands, each of the strands 6-50 nucleotides long;

    (ii) that is at least fifty percent double-stranded at 32°

    C.; and

    (iii) that has a terminal region on each end of its two separate strands and includes 1-4 modifying groups, each covalently attached to a different terminal region, said modifying groups being polycyclic moieties that do not have bulky portions that are non-planar,wherein said at least one double-stranded oligonucleotide additive is present in the reaction mixture at a concentration sufficient to suppress mispriming of an amplification reaction performed using the reaction mixture.

View all claims
  • 1 Assignment
Timeline View
Assignment View
    ×
    ×