Ultra-fast nucleic acid sequencing device and a method for making and using the same
First Claim
1. A system comprising:
- a substrate defining a pore and including four voltage carrying regions disposed along the pore;
a plurality of interconnects, each interconnect of the plurality of interconnects uniquely connected to a voltage carrying region of the four voltage carrying regions;
a plurality of power supplies in electrical communication with the plurality of interconnects, each power supply of the plurality of power supplies defining potential differences between a unique pair of voltage carrying regions selected from the four voltage carrying regions; and
a plurality of meters to measure a difference in electrical characteristics between a pair of voltage carrying regions of the unique pairs of voltage carrying regions, wherein the electrical characteristic is responsive to a base sequence of a nucleic acid.
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Accused Products
Abstract
A system and method employing at least one semiconductor device, or an arrangement of insulating and metal layers, having at least one detecting region which can include, for example, a recess or opening therein, for detecting a charge representative of a component of a polymer, such as a nucleic acid strand proximate to the detecting region, and a method for manufacturing such a semiconductor device. The system and method can thus be used for sequencing individual nucleotides or bases of ribonucleic acid (RNA) or deoxyribonucleic acid (DNA). The semiconductor device includes at least two doped regions, such as two n-typed regions implanted in a p-typed semiconductor layer or two p-typed regions implanted in an n-typed semiconductor layer. The detecting region permits a current to pass between the two doped regions in response to the presence of the component of the polymer, such as a base of a DNA or RNA strand. The current has characteristics representative of the component of the polymer, such as characteristics representative of the detected base of the DNA or RNA strand.
75 Citations
20 Claims
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1. A system comprising:
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a substrate defining a pore and including four voltage carrying regions disposed along the pore; a plurality of interconnects, each interconnect of the plurality of interconnects uniquely connected to a voltage carrying region of the four voltage carrying regions; a plurality of power supplies in electrical communication with the plurality of interconnects, each power supply of the plurality of power supplies defining potential differences between a unique pair of voltage carrying regions selected from the four voltage carrying regions; and a plurality of meters to measure a difference in electrical characteristics between a pair of voltage carrying regions of the unique pairs of voltage carrying regions, wherein the electrical characteristic is responsive to a base sequence of a nucleic acid. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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10. A system comprising:
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a substrate defining a pore and including a plurality of source or drain regions disposed at different positions separated longitudinally along the pore; a plurality of interconnects, each interconnect of the plurality of interconnects connected to a unique source or drain region of the plurality of source or drain regions; a plurality power supplies in electrical communication with the plurality of interconnects, each power supply of the plurality of power supplies defining potential differences between a unique pair of source or drain regions selected from the plurality of source or drain regions; and a plurality of current meters to measure a difference in current passing between pairs of source or drain regions, wherein the current is responsive to base sequence of a nucleic acid. - View Dependent Claims (11, 12, 13, 14, 15, 16)
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17. A method of nucleic acid sequencing comprising:
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applying a nucleic acid to a system comprising; a substrate defining a pore and including four voltage carrying regions disposed along the pore; a plurality of interconnects, each interconnect of the plurality of interconnects uniquely connected to a voltage carrying region of the four voltage carrying regions; a plurality of power supplies in electrical communication with the plurality of interconnects, each power supply of the plurality of power supplies defining potential differences between a unique pair of voltage carrying regions selected from the four voltage carrying regions; and a plurality of meters to measure a difference in electrical characteristics between a pair of voltage carrying regions of the unique pairs of voltage carrying regions, wherein the electrical characteristic is responsive to a base sequence of a nucleic acid; and measuring the electrical characteristic. - View Dependent Claims (18, 19, 20)
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Specification