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Innovation to assay mixing

  • US 9,766,213 B2
  • Filed: 10/02/2014
  • Issued: 09/19/2017
  • Est. Priority Date: 10/02/2013
  • Status: Active Grant
First Claim
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1. A method of calibrating a fluid delivery system, the method comprising:

  • providing a fluid delivery system connected to N input streams, wherein N is an integer greater than or equal to two, and wherein the fluid delivery system mixes together fluids from the N input streams to form an output stream;

    obtaining N test solutions, wherein each test solution comprises a dye having a characteristic wavelength of maximum absorbance (λ

    max);

    for each of the N test solutions,designating a wavelength λ

    test, wherein λ

    test is within the range from 90% of λ

    max to 110% of λ

    max, andproviding an input absorbance of the test solution, the input absorbance being the absorbance of the solution at λ

    test;

    injecting the N test solutions into the N input streams;

    mixing the N test solutions in the fluid delivery system;

    measuring the absorbance of the output stream at N wavelengths, the wavelengths being the λ

    tests designated for the test solutions, thereby obtaining an output absorbance for each test solution; and

    comparing the input absorbance with the output absorbance for each test solution, thereby calibrating the fluid delivery system.

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