Human placental collagen compositions, and methods of making and using the same

• US 9,775,886 B2
• Filed: 09/11/2012
• Issued: 10/03/2017
• Est. Priority Date: 10/06/2006
• Status: Active Grant

• Current Assignee: Celularity, Inc.
• Original Assignee: Celularity, Inc.
• Inventors: Bhatia, Mohit, Lugo, Chris, Ye, Qian, Edinger, James W.
• Primary Examiner(s): AUDET, MAURY A
• Application Number:
  US13/610,375
• Publication Number:
  US 20130071362A1
• Time in Patent Office: 1,848 Days
• Field of Search: None
• CPC Class Codes: A61K 2300/00 Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00
• CPC Class Codes: A61K 2800/92 Oral administration
• CPC Class Codes: A61K 38/39 Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin, cold insoluble globulin [CIG]
• CPC Class Codes: A61K 8/65 Collagen; Gelatin; Keratin; Derivatives or degradation products thereof
• CPC Class Codes: A61P 17/02 for treating wounds, ulcers, burns, scars, keloids, or the like
• CPC Class Codes: A61P 43/00 Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: A61Q 19/00 Preparations for care of the skin
• CPC Class Codes: A61Q 19/08 Anti-ageing preparations
• CPC Class Codes: C07K 1/02 in solution C07K1/003, C07K1/006 take precedence
• CPC Class Codes: C07K 1/34 by filtration, ultrafiltration or reverse osmosis
• CPC Class Codes: C07K 14/78 Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
• CPC Class Codes: G02B 2006/12147 Coupler
• CPC Class Codes: G02B 6/12004 Combinations of two or more optical elements
• CPC Class Codes: G02B 6/122 Basic optical elements, e.g. light-guiding paths
• CPC Class Codes: G02B 6/1228 Tapered waveguides, e.g. integrated spot-size transformers for coupling with fibres G02B6/305
• CPC Class Codes: G02B 6/124 Geodesic lenses or integrated gratings
• CPC Class Codes: G02B 6/2746 comprising non-reciprocal devices, e.g. isolators, FRM, circulators, quasi-isolators magneto-optic non-reciprocal devices G02F1/093
• CPC Class Codes: H01S 5/0064 Anti-reflection components, e.g. optical isolators
• CPC Class Codes: H01S 5/02326 Arrangements for relative positioning of laser diodes and optical components, e.g. grooves in the mount to fix optical fibres or lenses
• CPC Class Codes: H01S 5/026 Monolithically integrated components, e.g. waveguides, monitoring photo-detectors, drivers stabilisation of output H01S5/06
• CPC Class Codes: H01S 5/1032 : Coupling to elements comprising an optical axis that is not aligned with the optical axis of the active region
• CPC Class Codes: H01S 5/1035 : Forward coupled structures [DFC]
• CPC Class Codes: H01S 5/125 : Distributed Bragg reflector [DBR] lasers
• CPC Class Codes: H01S 5/187 : using Bragg reflection
• CPC Class Codes: H01S 5/2027 : Reflecting region or layer, parallel to the active layer, e.g. to modify propagation of the mode in the laser or to influence transverse modes
• CPC Class Codes: H01S 5/22 : having a ridge or stripe structure
• CPC Class Codes: H01S 5/3013 : AIIIBV compounds

Abstract

The present invention provides compositions comprising human placental telopeptide collagen, methods of preparing the compositions, methods of their use and kits comprising the compositions. The compositions, kits and methods are useful, for example, for augmenting or replacing tissue of a mammal.

Claims

1. A composition comprising placental extracellular matrix, wherein said composition comprises at least 80% collagen by dry weight, wherein said collagen comprises (i) between 74% and 90% Type I collagen, between 4% to 6% Type III collagen, and between 2% to 15% type IV collagen, (ii) no detectable amounts of fibronectin or laminin, and (iii) additional extracellular matrix components selected from the following groups:

(a) 4.7% elastin by dry weight and 0.28% glycosaminoglycans by dry weight;

(b) 3.2% elastin by dry weight and 0.34% glycosaminoglycans by dry weight;

(c) 4.4% elastin by dry weight and 0.38% glycosaminoglycans by dry weight; and

(d) 3.5% elastin by dry weight and 0.59% glycosaminoglycans by dry weight.

2. The composition of claim 1, wherein said placental collagen is human placental collagen.

3. The composition of claim 1, wherein said composition is shaped as a sheet.

4. The composition of claim 1, wherein said composition is shaped to fit to a site of a wound or injury.

5. The composition of claim 1, wherein said composition is formulated as a gel, a cream, a paste, a spray, an aerosol, or a solution.

6. The composition of claim 1, wherein said composition comprises a pharmacologically active agent.

7. The composition of claim 6, wherein said pharmacologically active agent comprises platelet-derived growth factor, insulin-like growth factor, epidermal growth factor, transforming growth factor beta, an angiogenesis factor, an antibiotic, an antifungal agent, a spermicidal agent, a hormone, an enzyme, or an enzyme inhibitor.

8. The composition of claim 1, wherein said composition is prepared by performing the following steps, in order:

a. macerating placental tissue;

b. suspending the tissue in an osmotic shock solution;

c. suspending the tissue in a solution that comprises detergent; and

d. washing the tissue.

9. The composition of claim 1, wherein the composition further comprises placental stem cells that are allogeneic to the subject.

10. The composition of claim 1, wherein the composition further comprises placental stem cells that are autologous to the subject.

11. The composition of claim 8, wherein the detergent comprises deoxycholate.

12. A method of treating a wound or burn of a subject comprising contacting the wound or burn with the composition of claim 1.

13. The method of claim 12, wherein said composition is used as a wound filler.

14. The method of claim 12, wherein said composition is applied directly to the site of the wound or burn.

15. A method of augmenting the skin of a subject in an area of the subject in need of augmenting, wherein said method comprises administering the composition of claim 1 to said area.

16. The method of claim 15, wherein said composition is administered by injection.

17. The method of claim 15, wherein said composition is administered to augment skin that requires augmentation as a result aging, trauma, disease, sickness, environmental factors, weight loss, or child birth.

18. The method of claim 15, wherein said composition is administered to augment skin that comprises wrinkles, frown lines, worry lines, crow'"'"'s feet, marionette lines, stretch marks, or scars.