Single molecule detection system and methods
First Claim
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1. A method of sequencing a nucleic acid, comprising:
- providing a detection apparatus comprisinga movable light coupler;
a waveguide comprising;
a core layer, anda first cladding layer,wherein at least one adapter site for the movable light coupler is formed in at least the first cladding layer; and
a light detector;
providing at least one nucleic acid molecule;
localizing the at least one nucleic acid molecule individually on the movable light coupler;
localizing at the adapter site the movable light coupler on which the at least one nucleic acid is localized;
generating an evanescent field introduced by a light wave propagating along the core layer;
coupling the evanescent field to the movable light coupler, and thereby forming an induced light field around the surface of the movable light coupler;
performing single molecule sequencing-by-synthesis of the at least one nucleic acid molecule, wherein the single molecule nucleic acid sequencing-by-synthesis leads to production of an emitted light correlated to the identity of at least one base in the nucleic acid;
detecting the emitted light with the detector, resulting in an output signal; and
processing the output signal to determine an identity of at least one base comprised by the nucleic acid.
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Abstract
Embodiments encompass a single-molecule detection system and methods of using the detection system to detect an object. Further, embodiments encompass a detection system comprising a movable light coupler, a waveguide, and a light detector. Embodiments further encompass methods of single-molecule detection, including methods of single-molecule nucleic acid sequencing.
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Citations
2 Claims
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1. A method of sequencing a nucleic acid, comprising:
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providing a detection apparatus comprising a movable light coupler; a waveguide comprising; a core layer, and a first cladding layer, wherein at least one adapter site for the movable light coupler is formed in at least the first cladding layer; and a light detector; providing at least one nucleic acid molecule; localizing the at least one nucleic acid molecule individually on the movable light coupler; localizing at the adapter site the movable light coupler on which the at least one nucleic acid is localized; generating an evanescent field introduced by a light wave propagating along the core layer; coupling the evanescent field to the movable light coupler, and thereby forming an induced light field around the surface of the movable light coupler; performing single molecule sequencing-by-synthesis of the at least one nucleic acid molecule, wherein the single molecule nucleic acid sequencing-by-synthesis leads to production of an emitted light correlated to the identity of at least one base in the nucleic acid; detecting the emitted light with the detector, resulting in an output signal; and processing the output signal to determine an identity of at least one base comprised by the nucleic acid. - View Dependent Claims (2)
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Specification