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Method for high-throughput AFLP-based polymorphism detection

  • US 9,777,324 B2
  • Filed: 04/22/2016
  • Issued: 10/03/2017
  • Est. Priority Date: 12/22/2005
  • Status: Active Grant
First Claim
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1. A method for genotyping of one or more polymorphisms, comprising:

  • pooling a first library of amplified DNA fragments and a second library of amplified DNA fragments to obtain a pooled DNA library, the amplified DNA fragments of the first library comprising DNA sequences amplified from a first sample by solution PCR and a first unique identifier sequence, the amplified DNA fragments of the second library comprising DNA sequences amplified from a second sample by solution PCR and a second unique identifier sequence, wherein the first and second unique identifier sequences indicate different sample origins of the first and second libraries;

    sequencing the pooled DNA library using high throughput sequencing technology, comprising amplifying on a solid support;

    clustering sequences of the first and second libraries of amplified DNA fragments; and

    determining dominant or co-dominant genotype(s) of one or more polymorphisms in the first and second libraries of amplified DNA fragments using the first and second unique identifier sequences.

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