×

Method for the detection and identification of methicillin-resistant Staphylococcus aureus

  • US 9,777,335 B2
  • Filed: 05/02/2006
  • Issued: 10/03/2017
  • Est. Priority Date: 06/04/2001
  • Status: Expired due to Term
First Claim
Patent Images

1. A method to detect the presence of an MREJ type vii methicillin-resistant Staphylococcus aureus (MRSA) strain comprising:

  • a.) performing an amplification reaction comprising contacting a sample to be analyzed for the presence of said MREJ type vii MRSA strain with a first amplification primer and a second amplification primer to generate a first amplicon if said MREJ type vii MRSA strain is present in said sample, said MREJ type vii MRSA strain including a staphylococcal cassette chromosome mec (SCCmec) element containing a mecA gene inserted into chromosomal DNA, said chromosomal DNA being orfX, thereby generating a polymorphic right extremity junction (MREJ) type vii sequence that comprises sequences from both the SCCmec element right extremity and orfX adjoining said right extremity;

    wherein said first primer is at least 10 nucleotides in length and specifically hybridizes at least under conditions of 50 mM KCl, 10 mM Tris-HCl (pH 9.0), 0.1% Triton X-100, 2.5 mM MgCl2 at 55°

    C. with an SCCmec element right extremity of an MREJ nucleic acid type vii sequence selected from the group consisting of;

    SEQ ID NO;

    165, SEQ ID NO;

    166, and the complements thereof,wherein said second primer is at least 10 nucleotides in length and specifically hybridizes at least under conditions of 50 mM KCl, 10 mM Tris-HCl (pH 9.0), 0.1% Triton X-100, 2.5 mM MgCl2 at 55°

    C. with orfX,wherein said first amplicon generated if said MREJ type vii MRSA strain is present in said sample comprises polymorphic right extremity junction (MREJ) type vii sequence and orfX sequence, including the junction of the two, and is indicative of the presence of MREJ type vii MRSA strain in said sample; and

    b.) detecting said first amplicon if present.

View all claims
  • 1 Assignment
Timeline View
Assignment View
    ×
    ×