Compositions and methods for detecting human Pegivirus 2 (HPgV-2)

• US 9,777,340 B2
• Filed: 06/26/2015
• Issued: 10/03/2017
• Est. Priority Date: 06/27/2014
• Status: Active Grant

• Current Assignee: Abbott Laboratories, Abbott Laboratories Incorporated (Abbott Laboratories), Regents of the University of California (University of California)
• Original Assignee: Abbott Laboratories, Regents of the University of California (University of California)
• Inventors: Chiu, Charles, Lee, Deanna, Berg, Michael, Dawson, George, Coller, Kelly, Cheng, Kevin, Hackett, John, Frankel, Matthew, Forberg, Kenn
• Primary Examiner(s): Blumel, Benjamin P
• Application Number:
  US14/752,262
• Publication Number:
  US 20150376722A1
• Time in Patent Office: 830 Days
• Field of Search: None
• CPC Class Codes: A61K 39/12 Viral antigens
• CPC Class Codes: A61K 49/085 conjugated systems
• CPC Class Codes: A61P 31/14 for RNA viruses
• CPC Class Codes: C07K 14/005 from viruses
• CPC Class Codes: C12N 2770/24021 Viruses as such, e.g. new isolates, mutants or their genomic sequences
• CPC Class Codes: C12N 2770/24022 New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
• CPC Class Codes: C12N 2770/24034 Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
• CPC Class Codes: C12Q 1/701 Specific hybridization probes
• CPC Class Codes: G01N 2333/183 Flaviviridae, e.g. pestivirus, mucosal disease virus, bovine viral diarrhoea virus, classical swine fever virus (hog cholera virus) or border disease virus
• CPC Class Codes: G01N 2469/20 Detection of antibodies in sample from host which are directed against antigens from microorganisms
• CPC Class Codes: G01N 33/56983 Viruses

Abstract

Provided herein are compositions, methods, and kits for detecting human Pegivirus 2 (HPgV-2). In certain embodiments, provided herein are HPgV-2 specific nucleic acid probes and primers, and methods for detecting HPgV-2 nucleic acid. In other embodiments, provided herein are HPgV-2 immunogenic composition compositions, methods of treating a subject with immunogenic HPgV-2 peptides, and methods of detecting HPgV-2 subject antibodies in a sample.

Claims

1. A method of detecting human Pegivirus 2 (HPgV-2) specific antibodies in a subject comprising:

a) contacting a sample from a subject suspected of containing an antibody to HPgV-2 with a HPgV-2 peptide, wherein said peptide specifically binds said antibody to form a complex, and

wherein said HPgV-2 peptide comprises an amino acid sequence selected from the group consisting of SEQ ID NOs;

86-99 and 100-218; and

b) detecting whether said antibody is present in said sample by detecting binding between said antibody and said peptide.

2. The method of claim 1, wherein said amino acid sequence is selected from the group consisting of SEQ ID NOs:

129-132.

3. The method of claim 1, wherein said amino acid sequence is selected from the group consisting of SEQ ID NOs:

163-166.

4. The method of claim 1, wherein said amino acid sequence is selected from the group consisting of SEQ ID NOs:

209-212.

5. The method of claim 1, wherein said amino acid sequence is selected from the group consisting of SEQ ID NOs:

213-218.

6. The method of claim 1, where said amino acid sequence is selected from the group consisting of SEQ ID NOs:

209-218.

7. The method of claim 1, wherein said HPgV-2 peptide comprises a label.

8. The method of claim 7, further comprising contacting said sample with a solid support comprising moieties that bind said label.

9. The method of claim 8, further comprising incubating said sample under conditions such that:

i) said HPgV-2 peptide specifically binds said antibody to form a complex, and ii) said complex binds to said solid support via said label binding at least one of said moieties.

10. The method of claim 9, further comprising washing said solid support.

11. The method of claim 10, further comprising adding to said sample a detectably labeled secondary antibody capable of binding the antibody in said complex.